Abstract

A novel method for electroformation of liposomes and phytosomes using copper electrode is described. Liposomes made at 2 V and 10 Hz AC field from L-α-egg-phosphatidylcholine (egg-PC), K. pneumoniae phosphatidylethanolamine, K. pneumoniae polar lipids and E. coli polar lipids on copper electrode were (777.9 ± 118.4), (370.2 ± 100.5), (825.3 ± 21.54), and (281.3 ± 42.3) nm in diameter, respectively. Giant vesicles were formed at 30 V and 10 Hz AC field from polar lipids of K. pneumoniae and E. coli were (106 ± 29.7) and (86 ± 24.3) µm in diameter, respectively. All liposomes were unilamellar as indicated by their unilamellar indices of 50 ± 2, had surface charge comparable to vesicles made from lipid(s) with similar composition and exhibited only 1–2 mol% of oxidized lipids. Cu concentration in the liposomal samples was <1.5 ppm for large unilamellar vesicles (LUVs) and ˂5 ppm for giant unilamellar vesicles (GUVs). The vesicles were stable for >15 d without loss of their size, charge, or unilamellarity. The method was successfully applied to prepare phytosomes from egg-PC and a phytochemical fraction of Dimorphocalyx glabellus, a medicinal plant with anti-diuretic properties. Phytosomes formed were 1000–1500 nm in diameter and exhibited altered fluorescence and absorbance properties compared to the unencapsulated phytochemical. Phytosomes with phytochemical: egg-PC ratio from 0.15 to 1.5 had encapsulation efficiency ranging 90–30%, respectively, and was stable for 1 month. Our method is easy, inexpensive and convenient that will prove to be useful for preparation of liposomes and phytosomes.

摘要

描述了一种使用铜电极电形成脂质体和植物体的新方法。在 2 V 和 10 Hz AC 场下由 L-α-蛋-磷脂酰胆碱 (egg-PC)、肺炎克雷伯氏菌磷脂酰乙醇胺、肺炎克雷伯菌极性脂质和铜电极上的大肠杆菌极性脂质制成的脂质体为 (777.9 ± 118.4)，直径分别为 (370.2 ± 100.5)、(825.3 ± 21.54) 和 (281.3 ± 42.3) nm。在 30 V 和 10 Hz AC 场下由肺炎克雷伯菌和大肠杆菌的极性脂质形成巨大囊泡直径分别为 (106 ± 29.7) 和 (86 ± 24.3) µm。所有脂质体都是单层的，如其单层指数为 50 ± 2 所示，具有与由相似组成的脂质制成的囊泡相当的表面电荷，并且仅表现出 1-2 mol% 的氧化脂质。脂质体样品中的铜浓度对于大单层囊泡 (LUV) 为 <1.5 ppm，对于巨大单层囊泡 (GUV) 为 ˂5 ppm。囊泡稳定 > 15 天而没有损失其大小、电荷或单层性。该方法已成功应用于以卵-PC和双形花萼的植物化学组分制备植物体，一种具有抗利尿特性的药用植物。与未封装的植物化学物质相比，形成的植物体直径为 1000-1500 nm，并表现出改变的荧光和吸光度特性。植物化学物质：鸡蛋-PC 比率为 0.15 至 1.5 的植物体的包封效率分别为 90-30%，并且可稳定保存 1 个月。我们的方法简单、便宜且方便，将证明可用于制备脂质体和植物体。