ABSTRACT

Thirty-two male Wistar albino rats were chosen to test the possible protective role of antioxidants of the edible seaweed Sargassum vulgare as a functional food additive to alleviate oxidative stress and toxicity associated with consumption of the artificial sweetener ‘aspartame (ASP)’. Biochemical and spleen histopathological analyses of the orally ASP-administrated rats, at a dose of 500 mg/kg for one week daily, showed different apoptotic and inflammatory patterns. Rats treated with ASP and then supplemented orally with the S. vulgare-MeOH extract, at a dose of 150 mg/kg for three consecutive weeks daily, showed significant positive reactions in all investigated assays related to ASP consumption. The protective and immune-stimulant efficacy of S. vulgare-MeOH extract, inferred from combating oxidative stress-induced lipid peroxidation, modulating the low levels of the endogenous antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and of the thyroid hormones T3 and T4, attenuating the elevated levels of apoptotic CASP-3 and inflammatory biomarkers TNF-α and IL-6, as well as heat shock proteins (Hsp70), can be most likely ascribed to the synergistic effect of its potent antioxidant phenolics (mainly gallic, ferulic, salicylic, and chlorogenic, and p-coumaric acids) and flavonoids (rutin, kaempferol, and hesperidin). Mechanism of action of these natural antioxidants was discussed.

摘要

选择了 32 只雄性 Wistar 白化病大鼠，以测试食用海藻马尾藻的抗氧化剂作为一种功能性食品添加剂的可能保护作用，以减轻与食用人造甜味剂“阿斯巴甜 (ASP)”相关的氧化应激和毒性。对口服 ASP 的大鼠进行生化和脾脏组织病理学分析，剂量为 500 mg/kg，每天 1 周，显示不同的凋亡和炎症模式。用 ASP 治疗的大鼠，然后口服补充S. vulgare -MeOH 提取物，剂量为 150 mg/kg，每天连续三周，在所有与 ASP 消耗相关的研究试验中显示出显着的阳性反应。S. vulgare的保护和免疫刺激功效-MeOH 提取物，从对抗氧化应激诱导的脂质过氧化作用推断，调节内源性抗氧化酶超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 以及甲状腺激素 T3 和 T4 的低水平，减弱凋亡 CASP 的升高水平- 3 和炎症生物标志物 TNF-α 和 IL-6 以及热休克蛋白 (Hsp70) 最有可能归因于其强效抗氧化酚类物质（主要是没食子、阿魏酸、水杨酸和绿原酸，以及p-香豆酸）和类黄酮（芦丁、山柰酚和橙皮苷）。讨论了这些天然抗氧化剂的作用机制。