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Edible Coatings Formulated with Antifungal GRAS Salts to Control Citrus Anthracnose Caused by Colletotrichum gloeosporioides and Preserve Postharvest Fruit Quality
Coatings ( IF 3.4 ) Pub Date : 2020-07-24 , DOI: 10.3390/coatings10080730
Victoria Martínez-Blay , María B. Pérez-Gago , Beatriz de la Fuente , Rosario Carbó , Lluís Palou

The in vitro antifungal activity of various generally recognized as safe (GRAS) salts against Colletotrichum gloeosporioides, the causal agent of citrus postharvest anthracnose, was evaluated as mycelial growth reduction on potato dextrose agar (PDA) dishes amended with salt aqueous solutions at different concentrations. The most effective treatments [0.2% ammonium carbonate (AC), 2% potassium sorbate (PS), 0.2% potassium carbonate (PC), 0.1% sodium methylparaben (SMP), 0.1% sodium ethylparaben (SEP), 2% sodium benzoate (SB) and 2% potassium silicate (PSi)] were selected as antifungal ingredients of composite edible coatings formulated with hydroxypropyl methylcellulose (HPMC)-beeswax (BW) matrixes. Stable coatings containing these salts were applied in in vivo curative experiments to “Nadorcott” mandarins and “Valencia” oranges artificially inoculated with C. gloeosporioides and those containing 2% PS, 2% SB and 2% PSi were the most effective to reduce anthracnose severity with respect to control fruit (up to 70% on mandarins). The effect of these selected coatings on the quality of non-inoculated and cold-stored “Valencia” oranges was determined after 28 and 56 days at 5 °C and 90% RH, followed by 7 days of shelf life at 20 °C. None of the coatings significantly reduced weight loss of coated oranges, but they modified their internal atmosphere, increasing the CO2 content. Overall, the coatings did not adversely affect the physicochemical and sensory attributes of the fruit.

中文翻译:

可食用涂料,配以抗真菌GRAS盐,可控制由炭疽菌引起的柑橘炭疽病,并保持采后果实品质

各种公认的安全(GRAS)盐对炭疽菌的体外抗真菌活性柑橘采后炭疽病的病原体,被评估为用不同浓度的盐水溶液修正的马铃薯葡萄糖琼脂(PDA)皿上菌丝体生长的减少。最有效的处理方法:[0.2%碳酸铵(AC),2%山梨酸钾(PS),0.2%碳酸钾(PC),0.1%对羟基苯甲酸甲酯(SMP),0.1%对羟基苯甲酸乙酯(SEP),2%苯甲酸钠( SB)和2%硅酸钾(PSi)]被选作由羟丙基甲基纤维素(HPMC)-蜂蜡(BW)基质配制的复合食用涂料的抗真菌成分。在体内治愈性实验中,将含有这些盐的稳定涂层应用于人工接种了C. gloeosporioides的“ Nadorcott”橘子和“ Valencia”橙子相对于对照水果,含2%PS,2%SB和2%PSi的炭黑对降低炭疽病的严重性最有效(普通话最高可达70%)。在5°C和90%RH下28天和56天后,然后在20°C下7天的保质期后,确定这些选择的涂层对未接种和冷藏的“巴伦西亚”橙品质的影响。没有一种涂层能显着降低涂层橙的重量损失,但是它们改变了内部气氛,增加了CO 2含量。总体而言,涂层对水果的物理化学和感官特性没有不利影响。
更新日期:2020-07-24
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