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Evaluating Temperature and Pressure Shock to Create Gynogenetic Cloned Progeny in Southern Flounder
North American Journal of Aquaculture ( IF 1 ) Pub Date : 2020-07-24 , DOI: 10.1002/naaq.10160
Elizabeth H. Silvy 1 , Todd D. Sink 1
Affiliation  

To address the needs of the growing aquaculture industry, we evaluated methodologies for creating gynogenetic female clones of Southern Flounder Paralichthys lethostigma. The primary objective was to determine which treatment to stop ejection of the second polar body, pressure or cold shock, would induce diploid genesis in ova that were activated with irradiated sperm and produce more viable offspring in Southern Flounder. The cold‐shock and pressure‐shock treatments with irradiated milt had similar egg‐activation rates and embryonic development rates of 1.3% (SE = 0.9) and 5.8% (SE = 2.7), respectively. However, pressure‐shock represents the most practical method for creating gynogenetic clones of the female.

中文翻译:

评估温度和压力冲击以在南部比目鱼中产生雌核发育的克隆后代

为了满足不断发展的水产养殖业的需求,我们评估了创建南方比目鱼Parahostythys lethostigma雌雄同体雌性克隆的方法。主要目的是确定哪种治疗方法可以停止第二极体的排出,压力或冷休克,从而诱导卵子中的二倍体发生,该二倍体由受精子激活并在南部比目鱼中产生更多的后代。辐照的冷冲击法和压力冲击法的卵活化率和胚胎发育率分别相似,分别为1.3%(SE = 0.9)和5.8%(SE = 2.7)。但是,压力冲击代表了创建雌性雌性生殖克隆的最实用方法。
更新日期:2020-07-24
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