Cisplatin resistance of non‐small‐cell lung cancer (NSCLC) needs to be well elucidated. RING finger protein (RNF38) has been proposed as a biomarker of NSCLC poor prognosis. However, its role in drug resistance in NSCLC is poorly understood. RNF38 expression was detected in normal lung epithelial cell and four NSCLC cell lines. RNF38 was stably overexpressed in A549 and H460 cells or silenced in H1975 and cisplatin‐resistant A549 cells (A549‐CDDP resistant) using lentiviral vectors. RNF38 expression levels were determined using quantitative real‐time polymerase chain reaction and western blotting analysis. Cell viability in response to different concentrations of cisplatin was evaluated by Cell Counting Kit‐8 assay. RNF38 expression levels were markedly elevated in NSCLC cells and cells harboring high RNF38 were less sensitive to cisplatin. Overexpression of RNF38 reduced, while RNF38 silencing increased the drug sensitivity of cisplatin in NSCLC cells. Cisplatin‐resistant cells expressed high RNF38 level. RNF38 silencing promoted cell apoptosis and enhanced the drug sensitivity of cisplatin in cisplatin‐resistant NSCLC cells. These findings indicate that RNF38 might induce cisplatin resistance of NSCLC cells via promoting cell apoptosis and RNF38 could be a novel target for rectify cisplatin resistance in NSCLC cases.

中文翻译：

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环指蛋白38诱导非小细胞肺癌顺铂耐药

需要很好地阐明非小细胞肺癌（NSCLC）的顺铂耐药性。环指蛋白 (RNF38) 已被提议作为 NSCLC 不良预后的生物标志物。然而，人们对其在 NSCLC 耐药中的作用知之甚少。在正常肺上皮细胞和四种 NSCLC 细胞系中检测到 RNF38 表达。RNF38 在 A549 和 H460 细胞中稳定过表达，或在 H1975 和抗顺铂 A549 细胞（A549-CDDP 抗性）中使用慢病毒载体沉默。使用定量实时聚合酶链反应和蛋白质印迹分析确定 RNF38 表达水平。通过细胞计数试剂盒-8 测定法评估响应于不同浓度顺铂的细胞活力。RNF38 表达水平在 NSCLC 细胞中显着升高，并且含有高 RNF38 的细胞对顺铂不太敏感。RNF38 的过表达降低，而 RNF38 沉默增加了顺铂在 NSCLC 细胞中的药物敏感性。顺铂抗性细胞表达高 RNF38 水平。RNF38沉默促进细胞凋亡并增强顺铂耐药NSCLC细胞对顺铂的药物敏感性。这些发现表明 RNF38 可能通过促进细胞凋亡来诱导 NSCLC 细胞对顺铂的耐药性，并且 RNF38 可能是纠正 NSCLC 病例中顺铂耐药的新靶点。