Quantitative determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma and urine with high accuracy and precision provides significant information to monitor the underlying etiology of several diseases. In this regard, liquid chromatography‐mass spectrometry is a good choice owing to its great selectivity and sensitivity. Additionally, the hybrid quadrupole–time of flight–mass spectrometer systems provides easy identification of target compounds with superior mass measurements. In this study, an analytical method has been developed for simple, accurate and simultaneous determination of linoleic acid, arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid in a short chromatographic analysis period. The developed method is suitable for the quantitative detection of these four compounds with detection limits ranging between 1.1–3.0 ng ml⁻¹ and its applicability was assessed in human urine and plasma samples. As a result, acceptable accuracy (between 83 and 111%) and good precision (<6%) were obtained for target compounds using matrix matching calibration strategy.

中文翻译：

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准确，灵敏地测定人血浆，尿液样品中的omega-6和omega-3多不饱和脂肪酸。

高精度和高精度地定量测定人血浆和尿液中的omega-6和omega-3多不饱和脂肪酸可为监测多种疾病的病因提供重要信息。在这方面，液相色谱-质谱法具有很高的选择性和灵敏度，因此是一个不错的选择。此外，混合四极杆-飞行时间-质谱仪系统可通过出色的质量测量轻松识别目标化合物。在这项研究中，开发了一种分析方法，可在短色谱分析期内简单，准确和同时测定亚油酸，花生四烯酸，二十二碳六烯酸和二十碳五烯酸。^-1及其适用性在人尿液和血浆样品中进行了评估。结果，使用基质匹配校准策略获得了目标化合物的可接受的准确性（83％至111％）和良好的准确性（<6％）。