Abstract Plant pathogenic fungi and bacteria cause large losses in the yield and quality of important crops. During the screening of bioactive plants against plant pathogens, we found that methanol extract of the aerial part of Rumex chinensis exhibited potent efficacy against various plant diseases. The methanol extract was effectively active against phytopathogenic fungi Blumeria graminis f. sp. hordei and Colletotrichum coccodes that cause plant diseases in vivo. n-Hexane and ethyl acetate layers obtained by liquid-liquid partitioning of the methanol extract strongly suppressed these fungal diseases with control values from 83 to 100% at 3000 µg/mL. These layers also inhibited the bacterial growth of Acidovorax avenae subsp. cattleyae and Ralstonia solanacearum. Notably, the n-hexane layer was found to reduce the development of tomato bacterial wilt in vivo. Six anthraquinones and two sterols were isolated from the aerial part of R. chinensis. Besides, the high-performance liquid chromatography (HPLC) was used for the quantitative analysis of anthraquinone contents in the active layers. The aerial part of R. chinensis was proved to be rich in active substances physcion, chrysophanol and emodin and their glycoside derivatives. Our findings suggested that the extracts and metabolites from the aerial part of R. chinensis could be used as potent active ingredients for developing botanical fungicide and bactericide to control plant diseases.

中文翻译：

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对各种植物病原体和瘤胃地上部分化学成分的体外和体内抗菌潜力。

摘要 植物病原真菌和细菌对重要作物的产量和品质造成巨大损失。在筛选抗植物病原体的生物活性植物时，我们发现瘤胃地上部分的甲醇提取物对各种植物病害表现出有效的功效。甲醇提取物对植物病原真菌 Blumeria graminis f 具有有效活性。sp. hordei 和 Colletotrichum coccodes 在体内引起植物病害。通过甲醇提取物的液-液分配获得的正己烷和乙酸乙酯层强烈抑制了这些真菌病害，控制值在 3000 µg/mL 时从 83% 到 100%。这些层还抑制了 Acidovorax avenae subsp. 的细菌生长。卡特兰和青枯病菌。尤其，发现正己烷层可减少体内番茄青枯病的发展。六种蒽醌类化合物和两种甾醇类化合物是从中华花的地上部分分离出来的。此外，高效液相色谱（HPLC）用于活性层中蒽醌含量的定量分析。实验证明，大黄的地上部分含有丰富的活性物质physcion、大黄酚和大黄素及其苷类衍生物。我们的研究结果表明，来自中华红地上部分的提取物和代谢物可用作开发植物杀菌剂和杀菌剂以控制植物病害的有效活性成分。高效液相色谱（HPLC）用于活性层中蒽醌含量的定量分析。实验证明，大黄的地上部分含有丰富的活性物质physcion、大黄酚和大黄素及其苷类衍生物。我们的研究结果表明，来自中华红地上部分的提取物和代谢物可用作开发植物杀菌剂和杀菌剂以控制植物病害的有效活性成分。高效液相色谱（HPLC）用于活性层中蒽醌含量的定量分析。实验证明，大黄的地上部分富含活性物质physcion、大黄酚和大黄素及其苷类衍生物。我们的研究结果表明，来自中华红地上部分的提取物和代谢物可用作开发植物杀菌剂和杀菌剂以控制植物病害的有效活性成分。