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Peptide array-based inhibition ELISA for evaluating antigenicity in infant formulas.
Journal of Bioscience and Bioengineering ( IF 2.8 ) Pub Date : 2020-07-24 , DOI: 10.1016/j.jbiosc.2020.06.010
Chisato Kubo 1 , Masaki Kurimoto 1 , Masayoshi Tanaka 2 , Hiroshi Ochi 1 , Fumiaki Abe 1 , Mina Okochi 2
Affiliation  

With increased awareness among consumers regarding food safety and security, food allergen control has become an indispensable requirement in the food industry. Although several methods for detecting allergens in food products are available, highly sensitive techniques are required. In this study, we developed a technique named as peptide array-based inhibition enzyme-linked immunosorbent assay (ELISA), Pep-iEIA, for evaluating antigenicity and detecting cow's milk antigen in infant formula products, using a peptide array consisting of a series of overlapping peptides found in allergenic milk proteins. Pep-iEIA was used to examine five cow's milk-based infant formulas with different degrees of hydrolyzation, and the assay offered both more sensitive detection and detailed analysis of remaining antigenic peptides in allergen compared to conventional ELISA. The antigenicity level of the allergenic peptides identified using Pep-iEIA was confirmed by surface plasmon resonance assay. We believe that Pep-iEIA will be highly useful for antigenicity evaluation of dairy products consumed by infants and patients with cow's milk allergy.



中文翻译:

基于肽阵列的抑制ELISA用于评估婴儿配方食品的抗原性。

随着消费者对食品安全性认识的提高,食品过敏原的控制已成为食品工业中不可或缺的要求。尽管有几种检测食品中过敏原的方法,但仍需要高度灵敏的技术。在这项研究中,我们开发了一种名为肽段抑制酶联免疫吸附测定(ELISA)的技术,Pep-iEIA,用于评估婴儿配方食品中的抗原性和检测牛奶抗原,使用一系列由乳蛋白中存在重叠的肽段。Pep-iEIA用于检查五种具有不同水解度的基于牛乳的婴儿配方食品,与传统的ELISA相比,该方法可以更灵敏地检测过敏原中剩余的抗原肽,并提供详细的分析。通过表面等离振子共振测定法确认了使用Pep-iEIA鉴定的变应原肽的抗原性水平。我们认为,Pep-iEIA对婴儿和患有牛奶过敏的患者食用的乳制品的抗原性评估将非常有用。

更新日期:2020-09-20
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