Cyanobacteria can grow photoautotrophically, producing a range of substances by absorbing sunlight and utilizing carbon dioxide, and can potentially be used as industrial microbes that have minimal sugar requirements. To evaluate this potential, we explored the possibility of l-glutamate production using the Synechocystis sp. PCC6803. The ybjL gene encoding the putative l-glutamate exporter from Escherichia coli was introduced, and l-glutamate production reached 2.3 g/L in 143 h (34°C, 100 μmol m⁻² s⁻¹). Then, we attempted to produce two flavor substances, (S)-linalool, a monoterpene alcohol, and the sesquiterpene (+)-valencene. The Synechocystis sp. PCC6803 strain in which the linalool synthase gene (LINS) from Actinidia arguta (AaLINS) was expressed under control of the tac promoter (GT0846K-P_tac-AaLINS) produced 11.4 mg/L (S)-linalool in 160 h (30°C, 50 μmol m⁻² s⁻¹). The strain in which AaLINS2 and the mutated farnesyl diphosphate synthase gene ispA∗ (S80F) from E. coli (GT0846K-P_psbA2-AaLINS-ispA∗) were expressed from the P_psbA2 promoter accumulated 11.6 mg/L (S)-linalool in 160 h. Genome analysis revealed that both strains had mutations in slr1270, suggesting that loss of Slr1270 function was necessary for high linalool accumulation. For sesquiterpene production, the valencene synthase gene from Callitropsis nootkatensis and the fernesyl diphosphate synthase (ispA) gene from E. coli were introduced, and the resultant strain produced 9.6 mg/L of (+)-valencene in 166 h (30°C, 50 μmol m⁻² s⁻¹). This study highlights the production efficiency of engineered cyanobacteria, providing insight into potential industrial applications.

蓝细菌可以自养生长，通过吸收阳光和利用二氧化碳产生一系列物质，并有可能被用作对糖的需求最少的工业微生物。为了评估这一潜力，我们探讨的可能性升谷氨酸盐生产中使用的蓝藻藻。PCC6803。引入编码来自大肠杆菌的假定的1-谷氨酸输出物的ybjL基因，并且在143小时（34℃，100μmolm ^-2  s ^-1）中1-谷氨酸的产量达到2.3g / L。然后，我们试图产生两个风味物质，（小号）-芳樟醇，单萜醇和倍半萜烯（+）-瓦伦烯。在蓝藻藻。PCC6803菌株，其中所述芳樟醇合酶基因（LINS）从软枣猕猴桃（AaLINS）的混合物在所述的控制下表达的tac启动子（GT0846K-P _tac启动- AaLINS）产生11.4毫克/升（小号）-linalool在160小时（30℃下50μmolm ^-2  s ^-1）。的菌株，其中AaLINS2和突变的法尼基二磷酸合酶基因ISPA *（S80F）从大肠杆菌（GT0846K-P _psbA2 - AaLINS -ispA＊）是在160小时内从P _psbA2启动子表达的，表达量为11.6 mg / L（S）-芳樟醇。基因组分析显示，这两个菌株均在slr1270中发生了突变，这表明Slr1270功能的丧失对于高的芳樟醇蓄积是必要的。为了生产倍半萜，引入了来自Callitropsis nootkatensis的valencene合酶基因和来自大肠杆菌的戊二酰二磷酸合酶（ispA）基因，并在166 h（30°C， 50μmolm ^-2  s ^-1）。这项研究突出了工程蓝细菌的生产效率，为潜在的工业应用提供了见识。