Nonspecific lipid transfer proteins (nsLTPs) play vital roles in lipid metabolism, cell apoptosis and biotic and abiotic stresses in plants. However, the distribution of nsLTPs in Arachis duranensis has not been fully characterized. In this study, we identified 64 nsLTP genes in A. duranensis (designated AdLTPs), which were classified into six subfamilies and randomly distributed along nine chromosomes. Tandem and segmental duplication events were detected in the evolution of AdLTPs. The K_s and ω values differed significantly between Types 1 and D subfamilies, and eight AdLTPs were under positive selection. The expression levels of AdLTPs were changed after salinity, PEG, low-temperature and ABA treatments. Three AdLTPs were associated with resistance to nematode infection, and DOF and WRI1 transcription factors may regulate the AdLTP response to nematode infection. Our results may provide valuable genomic information for the breeding of peanut cultivars that are resistant to biotic and abiotic stresses.

非特异性脂质转移蛋白 (nsLTPs) 在植物的脂质代谢、细胞凋亡以及生物和非生物胁迫中起着至关重要的作用。然而，nsLTPs 在花生中的分布尚未完全表征。在这项研究中，我们在A. duranensis（命名为AdLTPs）中鉴定了 64 个 nsLTP 基因，这些基因被分为六个亚科，并随机分布在九条染色体上。在AdLTPs的进化过程中检测到串联和节段重复事​​件。1 型和 D 型亚科之间的K _s和ω值显着不同，并且有 8 个AdLTP处于正选择状态。表达水平AdLTPs在盐度、PEG、低温和ABA处理后发生变化。三个 AdLTPs 与抗线虫感染有关，DOF 和 WRI1 转录因子可能调节 AdLTP 对线虫感染的反应。我们的研究结果可能为培育抗生物和非生物胁迫的花生品种提供有价值的基因组信息。