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Roles of six Hsp70 genes in virulence, cell wall integrity, antioxidant activity and multiple stress tolerance of Beauveria bassiana.
Fungal Genetics and Biology ( IF 3 ) Pub Date : 2020-07-24 , DOI: 10.1016/j.fgb.2020.103437
Jie Wang 1 , Jianwen Chen 2 , Yue Hu 3 , Sheng-Hua Ying 3 , Ming-Guang Feng 3
Affiliation  

Functions of most Hsp70-coding genes remain unknown in filamentous fungi. Here, we show important roles of six Hsp70 homologs (Hsp70a-f) in Beauveria bassiana, a filamentous fungal insect pathogen that serves as a main source of wide-spectrum fungal insecticides. In phylogeny, Hsp70b-e, Hsp70g and Hsp70h are distinct from eight other partners that share the same clade with 14 Hsp70 proteins in model yeast. Among the 14 Hsp70-coding genes in B. bassiana, eight (hsp70g/hsp70h/ssa/ssb/ssc/kar/sse/ssz) were individually undeletable perhaps due to an essentiality of each for the fungal viability. Moderate defects in growth and conidiation occurred in the absence of hsp70e or hsp70f (lsh1). The disruption of hsp70f resulted in a reduction of 43% in conidial heat (45 °C) tolerance and of 97% in virulence, and the two reductions decreased to 19–27% and 18–45% in the disruption mutants of hsp70a to hsp70d respectively. Conidial UVB resistance decreased more in the absence of hsp70e (33%) than of hsp70f (25%), hsp70c (19%) or hsp70d (15%). All of these disruption mutants were differentially compromised in both cell wall integrity and antioxidant activity and also in cellular tolerance to Na+, Zn2+, Mn2+, Cu2+ and/or Fe2+. Delayed acidification of submerged cultures and reduced secretion of organic acids occurred only in the absence of hsp70b or hsp70f. Our findings indicate that hsp70a-f are important for both host infection and multiple stress tolerance of B. bassiana, and functionally similar to or beyond the lsh1, ssb and ssz homologs characterized previously in some plant-pathogenic fungi.



中文翻译:

六个 Hsp70 基因在白僵菌毒力、细胞壁完整性、抗氧化活性和多重胁迫耐受性中的作用。

大多数 Hsp70 编码基因的功能在丝状真菌中仍然未知。在这里,我们展示了 6 个 Hsp70 同源物 (Hsp70a-f) 在球孢白僵菌中的重要作用,白僵菌是一种丝状真菌昆虫病原体,是广谱真菌杀虫剂的主要来源。在系统发育中,Hsp70b-e、Hsp70g 和 Hsp70h 与其他八个与模型酵母中的 14 个 Hsp70 蛋白共享相同进化枝的伙伴不同。在B. bassiana的 14 个 Hsp70 编码基因中,8 个 ( hsp70g/hsp70h/ssa/ssb/ssc/kar/sse/ssz ) 是单独不可删除的,这可能是由于每个基因对真菌生存力的重要性。在没有hsp70ehsp70f ( lsh1)。hsp70f的破坏导致分生孢子耐热 (45 °C) 耐受性降低 43%,毒力降低 97%,在hsp70ahsp70d的破坏突变体中,这两种降低分别降低至 19-27% 和 18-45%分别。在没有hsp70e (33%)的情况下,分生孢子 UVB 抗性比没有hsp70f (25%)、hsp70c (19%) 或hsp70d (15%) 降低得更多。所有这些破坏突变体在细胞壁完整性和抗氧化活性以及对 Na +、Zn 2+、Mn 2+、Cu 2+和/或 Fe 2+ 的细胞耐受性方面均受到不同程度的损害. 只有在不存在hsp70bhsp70f的情况下才发生浸没培养物的延迟酸化和有机酸分泌减少。我们的研究结果表明,HSP70A - ˚F是主机感染和多重抗逆性两个重要的球孢白僵菌和功能上类似于或超过lsh1SSBSSZ同源此前其特征在于一些植物病原真菌。

更新日期:2020-07-30
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