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Molecular interaction between methicillin-resistant Staphylococcus aureus (MRSA) and chicken breast reveals enhancement of pathogenesis and toxicity for food-borne outbreak.
Food Microbiology ( IF 5.3 ) Pub Date : 2020-07-24 , DOI: 10.1016/j.fm.2020.103602
Han Young Chung 1 , You-Tae Kim 2 , Joon-Gi Kwon 2 , Han Hyeok Im 1 , Duhyun Ko 1 , Ju-Hoon Lee 2 , Sang Ho Choi 1
Affiliation  

To study pathogenesis and toxicity of Staphylococcus aureus in foods, FORC_062 was isolated from a human blood sample and complete genome sequence has a type II SCCmec gene cluster and a type II toxin-antitoxin system, indicating an MRSA strain. Its mobile gene elements has many pathogenic genes involved in host infection, biofilm formation, and various enterotoxin and hemolysin genes. Clinical MRSA is often found in animal foods and ingestion of MRSA-contaminated foods causes human infection. Therefore, it is very important to understand the role of contaminated foods. To elucidate the interaction between clinical MRSA FORC_062 and raw chicken breast, transcriptome analysis was conducted, showing that gene expressions of amino acid biosynthesis and metabolism were specifically down-regulated, suggesting that the strain may import and utilize amino acids from the chicken breast, but not able to synthesize them. However, toxin gene expressions were up-regulated, suggesting that human infection of S. aureus via contaminated food may be more fatal. In addition, the contaminated foods enhance multiple-antibiotic resistance activities and virulence factors in this clinical MRSA. Consequently, MRSA-contaminated food may play a role as a nutritional reservoir as well as in enhancing factor for pathogenesis and toxicity of clinical MRSA for severe food-borne outbreaks.



中文翻译:

耐甲氧西林金黄色葡萄球菌(MRSA)与鸡胸肉之间的分子相互作用揭示了食源性暴发的发病机理和毒性增强。

为了研究食品中金黄色葡萄球菌的发病机理和毒性,从人类血液样本中分离出FORC_062,完整的基因组序列具有II型SCC mec基因簇和II型毒素-抗毒素系统,表明存在MRSA毒株。它的可移动基因元件具有许多与宿主感染,生物膜形成以及各种肠毒素和溶血素基因有关的致病基因。临床MRSA常在动物性食物中发现,摄入受MRSA污染的食物会引起人类感染。因此,了解受污染食品的作用非常重要。为了阐明临床MRSA FORC_062与生鸡胸肉之间的相互作用,进行了转录组分析,结果表明氨基酸生物合成和代谢的基因表达被特异性下调,这表明该菌株可能从鸡胸肉中导入和利用氨基酸,但是无法合成它们。然而,毒素基因表达上调,表明人类感染了经污染的食物引起的金黄色葡萄球菌可能更致命。此外,受污染的食物在该临床MRSA中增强了多种抗生素耐药性活性和毒力因子。因此,受MRSA污染的食品可能充当营养库,并增强临床MRSA对严重食源性暴发的发病机理和毒性的作用。

更新日期:2020-08-14
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