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Characterization of novel antigenic vaccine candidates for nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-07-24 , DOI: 10.1016/j.fsi.2020.07.024
Yanping Ma 1 , Le Hao 1 , Zhiling Liang 1 , Jiangyao Ma 1 , Hao Ke 1 , Huahua Kang 2 , Hongwei Yang 3 , Jing Wu 4 , Guoqing Feng 1 , Zhenxing Liu 1
Affiliation  

Streptococcus agalactiae is one of the important pathogens responsible for high mortality and economic losses of the tilapia industry worldwide. Based on ten serovars of S. agalactiae infection, subunit vaccine with conserved antigens is promising strategy corresponding stimulated long-term immunity and provides protection for animals against different serotypes of S. agalactiae. In the present study, eight proteins (AP, AL, LivK, ESAT6, essA, essB, essC and esaA) were selected from the S. agalactiae serotype Ia genome as immunogenic antigens with bioinformation and immune experiment assays. These recombinant proteins were successfully obtained through expression in Escherichia coli and the immunogenicity was assessed in tilapia challenge model. The results showed that the recombinant proteins caused high-level-specific antibodies production and high lysozyme activities, suggesting that the recombinant proteins induced specific humoral immune response and innate immune response of tilapia. The signficant increase were observed in the cytokines levels of TNF-α, IL-1β, IFN-γ, cc1, cc2 and immune-related genes levels of CD8α and MHC factors in the spleen and head kidney tissues, suggesting that the recombinant proteins induced immune response of tilapia through cytokines signal pathway and activated high cytotoxic T-lymphocyte (CTL) activity of tilapia. Furthermore, vaccinated tilapia conferred high levels of protection against challenge with a lethal dose of highly virulent serovar Ⅰa (highest RPS was 91.60% in AL and essC protein groups). Our results indicated that the eight recombinant proteins induced high level of immune responses and offered protection against S. agalactiae infection, could be potential subunit vaccine candidates.



中文翻译:

新型抗尼罗非鱼(尼罗罗非鱼)针对无乳链球菌感染的抗原疫苗的表征。

无乳链球菌是造成罗非鱼全球高死亡率和经济损失的重要病原体之一。基于无乳链球菌感染的十种血清型,具有保守抗原的亚单位疫苗是有望激发长期免疫力的有前途的策略,并为动物提供抵抗不同血清型无乳链球菌的保护。在本研究中,从S中选择了8种蛋白质(AP,AL,LivK,ESAT6,essA,essB,essC和esaA)。无乳血清型Ia基因组作为免疫原性抗原,具有生物信息和免疫实验方法。通过在大肠杆菌中表达成功获得了这些重组蛋白在罗非鱼攻击模型中评估了免疫原性。结果表明,重组蛋白引起高水平特异性抗体产生和高溶菌酶活性,表明重组蛋白诱导了罗非鱼的特异性体液免疫反应和先天免疫反应。TNF-αIL-1βIFN-γcc1cc2的细胞因子水平以及CD8αMHC的免疫相关基因水平显着增加在脾脏和头部肾脏组织中存在这种因子,提示重组蛋白通过细胞因子信号途径诱导罗非鱼的免疫反应,并激活罗非鱼的高细胞毒性T淋巴细胞(CTL)活性。此外,接种罗非鱼可给予致命剂量的高毒力血清Ⅰa高水平的抗攻击能力(AL和essC蛋白组的最高RPS为91.60%)。我们的结果表明,这八种重组蛋白可诱导高水平的免疫反应并提供针对无乳链球菌感染的保护,可能是潜在的亚单位疫苗候选物。

更新日期:2020-07-24
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