N6-methyladenosine (m6A) RNA methylation is one of the most abundant internal modifications on mRNAs and highly enriched within the brain. The demethylation of m6A is regulated by demethylases including fat-mass and obesity-associated protein (FTO) and AlkB homolog 5 (Alkbh5). FTO has been shown to play an important role in the brain, but little is known about the expression pattern and role of Alkbh5. Here, we investigated the expression profile of Alkbh5 in the developing mouse brain and its localization in the adult mouse brain. The results showed that Alkbh5 was widely detected throughout the mouse brain, with relatively high levels observed in the cerebellum and olfactory bulb of the adult mouse brain. Furthermore, Alkbh5 is mainly co-localized with neuronal marker NeuN, suggesting that it is primarily expressed in the neurons. Specifically, Alkbh5 could be found primarily in the nucleus of mouse neurons and cell lines. In addition, Alkbh5 protein decreased dramatically during brain development. Our findings detail the expression pattern and subcellular localization of Alkbh5 in the mouse brain. These results provide a neurobiological basis for the participation of Alkbh5 in the regulation of various brain functions, which might shed new light on further functional analysis of Alkbh5 and m6A in the central nervous system (CNS).

N6-甲基腺苷 (m6A) RNA 甲基化是 mRNA 上最丰富的内部修饰之一，在大脑中高度富集。m6A 的去甲基化受去甲基化酶的调控，包括脂肪量和肥胖相关蛋白 (FTO) 和 AlkB 同源物 5 (Alkbh5)。FTO 已被证明在大脑中发挥重要作用，但对 Alkbh5 的表达模式和作用知之甚少。在这里，我们研究了 Alkbh5 在发育中的小鼠大脑中的表达谱及其在成年小鼠大脑中的定位。结果表明，Alkbh5 在整个小鼠大脑中被广泛检测到，在成年小鼠大脑的小脑和嗅球中观察到相对较高的水平。此外，Alkbh5 主要与神经元标记 NeuN 共定位，表明它主要在神经元中表达。具体来说，Alkbh5 主要存在于小鼠神经元和细胞系的细胞核中。此外，Alkbh5 蛋白在大脑发育过程中急剧下降。我们的研究结果详细说明了 Alkbh5 在小鼠大脑中的表达模式和亚细胞定位。这些结果为Alkbh5参与各种脑功能的调节提供了神经生物学基础，这可能为Alkbh5和m6A在中枢神经系统（CNS）中的进一步功能分析提供新的思路。