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Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope.
Journal of Microbiology ( IF 3 ) Pub Date : 2020-07-24 , DOI: 10.1007/s12275-020-0060-1
Xiaoxia Wang 1, 2 , Jing Yang 2, 3, 4 , Yuyuan Huang 5 , Xiaomin Wu 6 , Licheng Wang 1 , Limei Han 1 , Sha Li 1 , Huan Li 1 , Xiaoying Fu 1 , Hai Chen 1 , Xiong Zhu 1
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Two aerobic, Gram-stain-positive, non-motile, non-sporulating coccoid strains, designated ZLJ0423T and ZLJ0321, were isolated from the faeces of Tibetan antelope (Pantholops hodgsonii). Their optimal temperature, NaCl concentration and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ZLJ0423T and ZLJ0321 were very similar to each other (99.8%) and had a sequence similarity of 97.0% with Georgenia satyanarayanai NBRC 107612T and Georgenia subflava CGMCC 1.12782T. Phylogenomic analysis based on 688 core genes indicated that these strains formed a clade with G. satyanarayanai NBRC 107612T and Georgenia wutianyii Z294. The predominant cellular fatty acids were anteiso-C15:0, anteiso-C15:1A and C16:0. The major menaquinone was MK-8(H4). The cell-wall amino acids consisted of alanine, lysine, glycine and aspartic acid, with lysine as the diagnostic diamino acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified lipids formed the polar lipid profile. The DNA G + C content of both isolates was 73.9 mol%. The digital DNA-DNA hybridization value between strains ZLJ0423T and ZLJ0321 was 91.2%, but their values with closely related species and other available type strains of the genus Georgenia were lower than the 70% threshold. On the basis of polyphasic taxonomic data, strains ZLJ0423T and ZLJ0321 represent a novel species within the genus Georgenia, for which the name Georgenia faecalis sp. nov. is proposed. The type strain is ZLJ0423T (= CGMCC 1.13681T = JCM 33470T).

中文翻译:

Georgia faecalis sp。十一月 与藏羚羊粪便隔离。

从藏羚羊(Pantholops hodgsonii)的粪便中分离出两个有氧,革兰氏染色阳性,不活动,不产孢子的类球菌菌株,分别命名为ZLJ0423 T和ZLJ0321 。它们的最佳生长温度,NaCl浓度和pH分别为28°C,0.5%(w / v)NaCl和pH 7.5。基于16S rRNA基因序列的系统进化分析表明,菌株ZLJ0423 T和ZLJ0321彼此非常相似(99.8%),与Georgia satayaarayanai NBRC 107612 TGeorgia subflava CGMCC 1.12782 T的序列相似性为97.0%。根据688个核心基因进行的系统进化分析表明,这些菌株形成了一个进化枝。G.satyanarayanai NBRC 107612 TGeorgenia wutianyii Z294。主要的细胞脂肪酸是前-C 15:0,前-C 15:1 A和C 16:0。主要的甲萘醌为MK-8(H 4)。细胞壁氨基酸由丙氨酸,赖氨酸,甘氨酸和天冬氨酸组成,赖氨酸作为诊断性二氨基酸。二磷脂酰甘油,磷脂酰甘油,磷脂酰肌醇,磷脂酰肌醇甘露糖苷和两种未鉴定的脂质形成极性脂质谱。两种分离物的DNA G + C含量为73.9mol%。ZLJ0423 T菌株间的数字DNA-DNA杂交价值。ZLJ0321和ZLJ0321为91.2%,但它们与密切相关的物种和其他可得的Georgania属菌株的均低于70%的阈值。上多相分类数据的基础上,菌株ZLJ0423 Ť和ZLJ0321代表属内一个新种Georgenia,为此,名称Georgenia肠球菌属。十一月 被提议。类型菌株为ZLJ0423 T(= CGMCC 1.13681 T = JCM 33470 T)。
更新日期:2020-07-24
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