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Molecular differences between arterial and venous grafts in the first year after coronary artery bypass grafting.
Histochemistry and Cell Biology ( IF 2.3 ) Pub Date : 2020-07-23 , DOI: 10.1007/s00418-020-01896-4
Christina Maria Steger 1 , Arndt Hartmann 2 , Ralf Joachim Rieker 2
Affiliation  

Despite commonly used for coronary artery bypass surgery, saphenous vein (SV) grafts have significantly lower patency rates in comparison to internal thoracic artery (ITA) grafts, which might be due to the structural characteristics of the vessel wall but also due to differences in oxidative stress adaptation and molecular signaling and regulation. This human post mortem study included a total of 150 human bypass grafts (75 SV grafts and 75 ITA grafts) obtained from 60 patients divided into five groups due to the time period of implantation: group 1: baseline group without grafting; group 2: 1 day; group 3: > 1 day–1 week; group 4: > 1 week–1 month; group 5: > 1 month–1 year. Pieces of 3 mm length were fixed with formaldehyde, dehydrated, wax embedded, cut into sections of 3 µm thickness, and histologically and immunohistochemically examined. Over the whole time period, we observed a lower neointima formation and a better preserved media in ITA grafts with a higher percentage of TNF-α, PDGFR-α, and VEGF-A in nearly all vessel wall layers, a higher amount of MMP-7, MMP-9, EGFR, and bFGF positive cells in SV grafts and a timely different peak not only between ITA and SV grafts but also within the various vessel wall layers of both graft types. Since most of the examined growth factors, growth factor receptors and cytokines are regulated by MAPKs, our results suggest an activation of different pathways in both vessel graft types immediately after bypass grafting.



中文翻译:

冠状动脉旁路移植术后第一年动脉和静脉移植物之间的分子差异。

尽管常用于冠状动脉搭桥手术,但与胸内动脉 (ITA) 移植物相比,大隐静脉 (SV) 移植物的通畅率要低得多,这可能是由于血管壁的结构特征以及氧化作用的差异所致。压力适应和分子信号和调节。该人体验尸研究包括从 60 名患者中获得的总共 150 个人体旁路移植物(75 个 SV 移植物和 75 个 ITA 移植物),由于植入时间不同分为五组: 组 1:未移植的基线组;第 2 组:1 天;第 3 组:> 1 天-1 周;第 4 组:> 1 周-1 个月;第 5 组:> 1 个月至 1 年。将 3 毫米长的碎片用甲醛固定,脱水,包蜡,切成 3 微米厚的部分,并进行组织学和免疫组织化学检查。在整个时间段内,我们观察到 ITA 移植物中新内膜形成率较低,中膜保存较好,几乎所有血管壁层中 TNF-α、PDGFR-α 和 VEGF-A 的百分比较高,MMP-A 的含量较高。 7、SV 移植物中的 MMP-9、EGFR 和 bFGF 阳性细胞,并且不仅在 ITA 和 SV 移植物之间而且在两种移植物类型的各种血管壁层内都有及时的不同峰值。由于大多数检查的生长因子、生长因子受体和细胞因子受 MAPK 调节,我们的结果表明在旁路移植后两种血管移植类型中的不同途径立即被激活。和 VEGF-A 在几乎所有的血管壁层中,在 SV 移植物中 MMP-7、MMP-9、EGFR 和 bFGF 阳性细胞的数量更高,并且不仅在 ITA 和 SV 移植物之间而且在各种血管内都有及时的不同峰值两种移植物类型的壁层。由于大多数检查的生长因子、生长因子受体和细胞因子受 MAPK 调节,我们的结果表明在旁路移植后两种血管移植类型中的不同途径立即被激活。和 VEGF-A 在几乎所有的血管壁层中,在 SV 移植物中 MMP-7、MMP-9、EGFR 和 bFGF 阳性细胞的数量更高,并且不仅在 ITA 和 SV 移植物之间而且在各种血管内都有及时的不同峰值两种移植物类型的壁层。由于大多数检查的生长因子、生长因子受体和细胞因子受 MAPK 调节,我们的结果表明在旁路移植后两种血管移植类型中的不同途径立即被激活。

更新日期:2020-07-24
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