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Calcium-responsive hydrogels enabled by inducible protein–protein interactions
Polymer Chemistry ( IF 4.6 ) Pub Date : 2020-07-23 , DOI: 10.1039/d0py00423e
Jiren Luo 1, 2, 3, 4 , Fei Sun 1, 2, 3, 4, 5
Affiliation  

The inherent complexity of cell–matrix interactions calls for smart materials that can actively sense and respond to environmental stimuli with physiological relevance. Here we demonstrate the creation of entirely protein-based Ca2+-responsive hydrogels by covalently assembling calmodulin (CaM) and its cognate ligand, M13-peptide, using SpyTag/SpyCatcher chemistry. The resulting protein hydrogels comprising CaM and M13 exhibited mechanics and stability dependent on Ca2+, an important second messenger in biology, as well as suitability for 3D cell culturing. These results demonstrate the feasibility of using inducible protein–protein interactions to design biomaterials with dynamic properties, providing new opportunities for materials biology.

中文翻译:

钙诱导性水凝胶可通过诱导性蛋白质-蛋白质相互作用实现

细胞与基质相互作用的内在复杂性要求使用能够主动感知并响应具有生理相关性的环境刺激的智能材料。在这里,我们通过使用SpyTag / SpyCatcher化学方法共价组装钙调蛋白(CaM)及其同源配体M13肽,证明了完全基于蛋白质的Ca 2+响应水凝胶的创建。所得包含CaM和M13的蛋白质水凝胶显示出取决于Ca 2+的力学和稳定性,Ca 2+是生物学中的重要第二信使,并且适合3D细胞培养。这些结果证明了使用诱导型蛋白质相互作用来设计具有动态特性的生物材料的可行性,这为材料生物学提供了新的机会。
更新日期:2020-08-11
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