Long noncoding RNAs (lncRNAs) contribute toward regulating gene expression and cell differentiation and may be involved in the pathogenesis of several diseases. The objective of this study was to determine the expression patterns of lncRNAs in bone marrow mesenchymal stem cells (BMSCs) derived from patients with osteoporotic fractures and their relevance to osteogenic function. The BMSCs were isolated from the femoral head of patients with hip fractures (FRX) and controls with osteoarthritis (OA). We found 74 differentially expressed genes between FRX and OA, of which 33 were of the lncRNA type. Among them, 52 genes (20 lncRNAs) were replicated in another independent dataset. The differentially expressed lncRNAs were over-represented among those correlated with differentially expressed protein-coding genes. In addition, the comparison of pre- and post-differentiated paired samples revealed 163 differentially expressed genes, of which 99 were of the lncRNA type. Among them, the overexpression of LINC00341 induced an upregulation of typical osteoblastic genes. In conclusion, the analysis of lncRNA expression in BMSCs shows specific patterns in patients with osteoporotic fractures, as well as changes associated with osteogenic differentiation. The regulation of bone genes through lncRNAs might bring new opportunities for designing bone anabolic therapies in systemic and localized bone disorders.

中文翻译：

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长的非编码RNA作为骨质疏松症中的骨髓干细胞调节剂。

长非编码RNA（lncRNA）有助于调节基因表达和细胞分化，并可能参与多种疾病的发病机制。这项研究的目的是确定lncRNA在骨质疏松性骨折患者骨髓间充质干细胞（BMSCs）中的表达模式及其与成骨功能的相关性。从患有髋部骨折（FRX）和骨关节炎（OA）的患者的股骨头中分离出BMSC。我们发现FRX和OA之间有74个差异表达基因，其中33个是lncRNA类型。其中，在另一个独立的数据集中复制了52个基因（20个lncRNA）。在与差异表达的蛋白质编码基因相关的那些中，差异表达的lncRNA的表达过高。此外，分化前和分化后配对样品的比较显示了163个差异表达基因，其中99个是lncRNA类型。其中，过度表达LINC00341诱导典型的成骨细胞基因上调。总之，对BMSCs中lncRNA表达的分析显示出骨质疏松性骨折患者的特定模式，以及与成骨分化相关的变化。通过lncRNA调节骨骼基因可能为设计全身性和局部性骨骼疾病的骨合成代谢疗法提供新的机会。