The experimental objective was to investigate the impact of xylanase on the bioavailability of energy, oxidative status, and gut function of growing pigs fed a diet high in insoluble fiber and given a longer adaptation time than typically reported. Three replicates of 20 gilts with an initial body weight (BW) of 25.43 ± 0.88 kg were blocked by BW, individually housed, and randomly assigned to 1 of 4 dietary treatments: a low-fiber control (LF) with 7.5% neutral detergent fiber (NDF), a 30% corn bran without solubles high-fiber control (HF; 21.9% NDF), HF + 100 mg/kg xylanase (HF+XY; Econase XT 25P), and HF + 50 mg/kg arabinoxylan-oligosaccharide (HF+AX). Gilts were fed ad libitum for 36 d across 2 dietary phases. Pigs and feeders were weighed on d 0, 14, 27 and 36. On d 36, pigs were housed in metabolism crates for a 10-d period, limit fed (80% of average ad libitum intake) and feces and urine were collected the last 72 h to determine digestible energy (DE) and metabolizable energy (ME). On d 46, serum and ileal and colonic tissue were collected. Data were analyzed as a linear mixed model with block and replication as random effects, and treatment, time, and treatment × time as fixed effects. There was a significant treatment × time interaction for BW, average daily gain (ADG), and gain to feed (G:F; P<0.001). By design, BW at d 0 did not differ, at d 14 pigs fed LF were 3.5% heavier, and pigs fed HF+XY, when compared to HF, were 4% and 4.2% heavier at d 27 and d 36, respectively (P <0.001). From d 14 to 27 and d 27 to 36, when compared to HF, HF+XY improved ADG by 12.4% and 10.7% and G:F by 13.8% and 8.8%, respectively (P<0.05). Compared to LF, HF decreased DE and ME by 0.51 and 0.42 Mcal/kg, respectively, but xylanase partially mitigated that effect increasing DE and ME by 0.15 and 0.12 Mcal/kg, over HF, respectively (P<0.05). Pigs fed HF+XY had increased total antioxidant capacity in the serum and ileum (P<0.05) and tended to have less circulating malondialdehyde (P=0.098). Pigs fed LF had increased ileal villus height, and HF+XY and HF+AX had shallower intestinal crypts (P<0.001). Pigs fed HF+XY had increased ileal messenger ribonucleic acid abundance of claudin 4 and occludin (P<0.05). Xylanase, but not AX, improved the growth performance of pigs fed insoluble corn-based fiber. This was likely a result of the observed increase in ME, improved antioxidant capacity and enhanced gut barrier integrity, but it may require increased adaptation time to elicit this response.

中文翻译：

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木聚糖酶增加了纤维的能量贡献，并改善了饲喂不溶性玉米基纤维的生长猪的氧化状态，肠屏障完整性和生长性能。

实验目的是研究木聚糖酶对日粮中不溶性纤维含量高且适应时间比通常报道的长的生长猪的能量，氧化状态和肠道功能的生物利用度的影响。初始体重（BW）为25.43±0.88公斤的20头母猪的三份重复试验被BW阻断，单独饲养，并随机分配给4种饮食疗法中的一种：低纤维对照（LF）和7.5％中性洗涤剂纤维（NDF），不含可溶性高纤维对照（HF; 21.9％NDF），HF + 100 mg / kg木聚糖酶（HF + XY; Econase XT 25P）和HF + 50 mg / kg阿拉伯木聚糖低聚糖的30％玉米麸（HF + AX）。在两个饮食阶段中，母猪自由喂食36天。在第0、14、27和36天称重猪和喂食器。在第36天，将猪放在代谢箱中饲养10天，限饲（平均自由采食量的80％），最后72小时收集粪便和尿液以确定可消化能（DE）和可代谢能（ME）。在第46天，收集血清，回肠和结肠组织。数据分析为线性混合模型，其中阻断和复制为随机效应，治疗，时间和治疗×时间为固定效应。体重，时间平均日增重（ADG）和饲料增重（G：F； P <0.001）之间存在显着的治疗×时间相互作用。根据设计，在d 0时的体重没有差异，在d 14时饲喂LF的猪重3.5％，而与HF相比，饲喂HF + XY的猪在d 27和d 36时分别重4％和4.2％（ P <0.001）。与HF相比，从d 14至27和d 27至36，HF + XY使ADG分别提高12.4％和10.7％，以及G：F分别提高13.8％和8.8％（P <0.05）。与LF相比 HF使DE和ME分别比HF降低0.51和0.42 Mcal / kg，但木聚糖酶部分缓解了这种作用，使DE和ME分别提高0.15和0.12 Mcal / kg（P <0.05）。饲喂HF + XY的猪的血清和回肠中的总抗氧化能力增强（P <0.05），并且循环丙二醛含量降低（P = 0.098）。饲喂LF的猪回肠绒毛高度增加，而HF + XY和HF + AX的肠隐窝变浅（P <0.001）。饲喂HF + XY的猪回肠信使核糖核酸4的含量和闭合蛋白的含量增加（P <0.05）。木聚糖酶（而不是AX）改善了饲喂不溶性玉米基纤维的猪的生长性能。这可能是由于观察到的ME增加，抗氧化能力提高和肠屏障完整性提高的结果，但是可能需要增加适应时间才能引起这种反应。