SummaryThe aim of the study was to investigate the continuous changing pattern of H4K12 acetylation, and the expression levels of histone acetyltransferases (HATs) and histone deacetyltransferases (HDACs) in mouse oocytes during meiosis and after parthenogenetic activation (PA). The immunofluorescence results showed hyperacetylation of lysine-12 on histone H4 (H4K12) in the germinal vesicle (GV) oocytes that then decreased during germinal vesicle breakdown (GVBD), and disappeared in metaphase II (MII). However, it reappeared in the early 1-cell embryos derived after 4 h of PA. The expression levels of some selected HATs and HDACs also validated the changing pattern of H4K12 acetylation during meiosis and PA. In conclusion, H4K12 is deacetylated in GVBD and MII, and re-hyperacetylated after PA.

中文翻译：

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减数分裂成熟期间和小鼠卵母细胞孤雌生殖激活后 H4K12 乙酰化的动态变化

摘要本研究的目的是研究小鼠卵母细胞在减数分裂和孤雌生殖激活 (PA) 后 H4K12 乙酰化的持续变化模式，以及组蛋白乙酰转移酶 (HAT) 和组蛋白去乙酰转移酶 (HDAC) 的表达水平。免疫荧光结果显示，在生殖囊泡 (GV) 卵母细胞中，组蛋白 H4 (H4K12) 上赖氨酸 12 的高度乙酰化，然后在生殖囊泡破裂 (GVBD) 期间降低，并在中期 II (MII) 中消失。然而，它重新出现在 PA 4 小时后衍生的早期 1 细胞胚胎中。一些选定的 HAT 和 HDAC 的表达水平也验证了减数分裂和 PA 期间 H4K12 乙酰化的变化模式。总之，H4K12 在 GVBD 和 MII 中脱乙酰化，并在 PA 后重新高乙酰化。