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The effects of foliar application of melatonin on some physiological and biochemical characteristics and expression of fatty acid desaturase gene in pistachio seedlings (Pistacia vera L.) under freezing stress
Journal of Plant Interactions ( IF 3.2 ) Pub Date : 2020-07-23 , DOI: 10.1080/17429145.2020.1781271
Adeleh Barand 1 , Fatemeh Nasibi 1, 2 , Khosrow Manouchehri Kalantari 1 , Mohammad Moradi 3
Affiliation  

ABSTRACT

In this study, the impacts of exogenous melatonin on freezing stress mitigation in pistachio (Pistacia vera L.) seedlings (30-days old) were investigated. Our results showed that in pistachio seedlings under freezing stress antioxidant enzyme activity, malondialdehyde (MDA) hydrogen peroxide (H2O2), electrolyte leakage, chlorophyll degradation, proline and soluble sugar and γ-aminobutyric acid (GABA) content increased. Phenolic component, phenylalanine ammonia lyase (PAL) activity and the expression of fatty acid desaturase (FAD2) gene increased whilst ascorbate and glutathione concentration significantly decreased in comparison with control. In this research, application of melatonin significantly reduced the antioxidant enzyme activity, MDA, H2O2, chlorophyll degradation, GABA concentration, soluble sugar, and proline contents under stress condition. In addition electrolyte leakage, PAL activity and transcripts of FAD2 gene markedly decreased when the leaves of pistachio seedlings sprayed with melatonin. The results of this study showed that MEL could alleviate the oxidative damages induced by freezing stress and to maintain the structure and mobility of cell membrane in pistachio seedlings under stress conditions. Considering that MEL is an inexpensive and safe bio-stimulator, it seems that MEL is a potential candidate that could be applied used to increase the resistant pistachio trees against variety of biotic and abiotic stresses in the field conditions.



中文翻译:

叶面褪黑素对冷冻胁迫下开心果幼苗某些生理生化特性及脂肪酸去饱和酶基因表达的影响

摘要

在这项研究中,研究了外源褪黑素对开心果(Pistacia vera L.)幼苗(30天大)的冷冻胁迫缓解的影响。我们的结果表明,开心果幼苗在冷冻胁迫下具有抗氧化酶活性,丙二醛(MDA)过氧化氢(H 2 O 2),电解质泄漏,叶绿​​素降解,脯氨酸和可溶性糖以及γ-氨基丁酸(GABA)含量均增加。酚类成分,苯丙氨酸氨裂合酶(PAL)活性和脂肪酸去饱和酶(FAD2)的表达与对照相比,基因增加,而抗坏血酸和谷胱甘肽浓度显着降低。在这项研究中,褪黑激素的应用显着降低了在胁迫条件下的抗氧化酶活性,MDA,H 2 O 2,叶绿素降解,GABA浓度,可溶性糖和脯氨酸含量。另外电解质泄漏,PAL活性和FAD2的转录本开心果幼苗叶片喷洒褪黑激素后,该基因显着降低。研究结果表明,MEL在胁迫条件下可以减轻开心果幼苗因冰冻胁迫而引起的氧化损伤,并维持其细胞膜的结构和迁移。考虑到MEL是一种便宜且安全的生物刺激剂,MEL似乎是一种潜在的候选物,可用于提高开心果树对田间条件下各种生物和非生物胁迫的抗性。

更新日期:2020-07-23
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