ABSTRACT To facilitate the cell-based experiment for pulsed electromagnetic field biological effect study, a novel TEM-cell-integrated CO2 incubator was developed. The integrated experimental system could simultaneously meet the requirement of standard cell culture condition and the various Transient Electromagnetic Field (TEF) exposure, which made it possible to study the relationship between different electromagnetic pulse exposure and the cellular responses in a reliable way. During the research, a comparison experiment was carried out to evaluate the necessity of the integrated incubator system: firstly, two different types of cell lines, which are the human prostate cancer cell line (PC3) and the pancreatic β cell line (MIN6) were chosen and exposed in the TEM-cell which located in the open area and the integrated system, respectively, with the same EFT radiation conditions; then, the cells’ viability, the cellular ROS level and the mitochondrial membrane potential (MMP) were detected, respectively. The results showed that in the same parameter of the EFT radiation, the processes of the cells had a significant difference and even opposite in the incubator and open area, and all the results could be reproducible. The phenomenon indicated the stability of the TEM-cell-integrated CO2 incubator, and also demonstrated the necessity to strictly control the cell culture condition when carrying out the precise mechanism study of the TEF bioresponse at the cellular levels.

中文翻译：

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用于基于细胞的瞬态电磁场生物效应研究的 TEM 细胞集成 CO2 培养箱的开发

摘要 为了促进基于细胞的脉冲电磁场​​生物效应研究实验，开发了一种新型 TEM 细胞集成 CO2 培养箱。集成的实验系统可以同时满足标准细胞培养条件和各种瞬态电磁场（TEF）暴露的要求，这使得以可靠的方式研究不同电磁脉冲暴露与细胞反应之间的关系成为可能。在研究过程中，进行了对比实验以评估集成培养箱系统的必要性：首先，两种不同类型的细胞系，即人前列腺癌细胞系（PC3）和胰腺β细胞系（MIN6）选择并暴露在分别位于开放区域和集成系统的 TEM 单元中，具有相同的 EFT 辐射条件；然后，分别检测细胞活力、细胞活性氧水平和线粒体膜电位（MMP）。结果表明，在相同的EFT辐射参数下，培养箱和开放区域的细胞过程有显着差异甚至相反，所有结果均具有可重复性。这一现象表明了 TEM 细胞集成 CO2 培养箱的稳定性，也证明了在细胞水平上进行 TEF 生物反应的精确机制研究时严格控制细胞培养条件的必要性。结果表明，在相同的EFT辐射参数下，培养箱和开放区域的细胞过程有显着差异甚至相反，所有结果均具有可重复性。这一现象表明了 TEM 细胞集成 CO2 培养箱的稳定性，也证明了在细胞水平上进行 TEF 生物反应的精确机制研究时严格控制细胞培养条件的必要性。结果表明，在相同的EFT辐射参数下，培养箱和开放区域的细胞过程有显着差异甚至相反，所有结果均具有可重复性。这一现象表明了 TEM 细胞集成 CO2 培养箱的稳定性，也证明了在细胞水平上进行 TEF 生物反应的精确机制研究时严格控制细胞培养条件的必要性。