Systemic acquired acclimation (SAA) is a key biological process essential for plant survival under conditions of abiotic stress. SAA was recently shown to be controlled by a rapid systemic signaling mechanism termed the reactive oxygen species (ROS) wave in Arabidopsis (Arabidopsis thaliana). MYB30 is a key transcriptional regulator mediating many different biological processes. MYB30 was found to act downstream of the ROS wave in systemic tissues of Arabidopsis in response to local high light (HL) stress treatment. However, the function of MYB30 in systemic signaling and SAA is unknown. To determine the relationship among MYB30, the ROS wave, and systemic acclimation in Arabidopsis, the SAA response to HL stress of myb30 mutants and wild-type plants was determined. Although myb30 plants were found to display enhanced rates of ROS wave propagation and their local tissues acclimated to the HL stress, they were deficient in SAA to HL stress. Compared to wild type, the systemic transcriptomic response of myb30 plants was also deficient, lacking in the expression of over 3,500 transcripts. A putative set of 150 core transcripts directly associated with MYB30 function during HL stress was determined. Our study identifies MYB30 as a key regulator that links systemic ROS signaling with systemic transcriptomic responses, SAA, and plant acclimation to HL stress. In addition, it demonstrates that plant acclimation and systemic ROS signaling are interlinked and that the lack of systemic acclimation drives systemic ROS signaling to occur at faster rates, suggesting a feedback mechanism (potentially involving MYB30) between these two processes.

系统获得性驯化（SAA）是非生物胁迫条件下植物生存所必需的关键生物过程。最近表明，SAA 是由拟南芥 ( Arabidopsis thaliana )中称为活性氧 (ROS) 波的快速系统信号机制控制的。MYB30 是介导许多不同生物过程的关键转录调节因子。研究发现，MYB30 在拟南芥全身组织中的 ROS 波下游发挥作用，以响应局部高光 (HL) 应激处理。然而，MYB30 在系统信号传导和 SAA 中的功能尚不清楚。为了确定拟南芥中 MYB30、ROS 波和系统驯化之间的关系，确定了myb30突变体和野生型植物对 HL 胁迫的 SAA 反应。尽管发现myb30植物表现出增强的 ROS 波传播速率并且其局部组织适应了 HL 胁迫，但它们在 SAA 到 HL 胁迫方面存在缺陷。与野生型相比，myb30植物的系统转录组反应也有缺陷，缺乏超过 3,500 个转录本的表达。确定了一组假定的 150 个核心转录本，这些转录本与 HL 应激期间的 MYB30 功能直接相关。我们的研究确定 MYB30 是一个关键调节因子，它将系统 ROS 信号传导与系统转录组反应、SAA 以及植物对 HL 胁迫的适应联系起来。此外，它表明植物驯化和系统性 ROS 信号传导是相互关联的，并且缺乏系统性驯化会导致系统性 ROS 信号以更快的速度发生，表明这两个过程之间存在反馈机制（可能涉及 MYB30）。