Marine algae are important sources of biological substances. Polysaccharides and oil in algae have a broad range of biological activities. The aim of our study was to develop a novel method for the extraction of polysaccharides and oil from Schizochytrium limacinum by enzyme‐assisted three‐phase partitioning. S. limacinum was pretreated by protamex hydrolysis for 2 hr, at pH 3.0, temperature of 45°C, and enzyme concentration of 2.0%. Response surface was used to analyze the three‐phase extraction and Box–Behnken design was employed to model algae oil and polysaccharides. The following optimal conditions were obtained by global optimization: 34% mass fraction of ammonium sulfate, 1:2.4 ratio of slurry to t‐butanol, 41 min time, 40°C temperature, 35.69% oil yield, and 5.16% polysaccharides yield. The polysaccharides exhibited potential antioxidant activity in DPPH, ABTS, and reducibility assay. The results indicated that the algal polysaccharides may be used as a natural antioxidant in food.

中文翻译：

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通过酶辅助三相分配同时优化甘草裂殖藻中油和多糖的全局优化

海藻是生物物质的重要来源。藻类中的多糖和油具有广泛的生物活性。我们的研究目的是开发一种新的酶辅助三相分配技术从甜酒裂殖壶菌中提取多糖和油的方法。通过Protamex水解在pH 3.0，温度为45°C和酶浓度为2.0％的条件下预处理2小时，从而对葡萄球菌进行了预处理。响应面用于分析三相萃取，Box-Behnken设计用于模拟藻类油和多糖。通过全局优化获得了以下最佳条件：硫酸铵的质量分数为34％，浆料与吨的比例为1：2.4丁醇，41分钟，40°C温度，35.69％的油收率，和5.16％的多糖收率。多糖在DPPH，ABTS和还原性测定中显示出潜在的抗氧化活性。结果表明藻类多糖可用作食品中的天然抗氧化剂。