Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects, but it is difficult to distinguish between them in immune reactions. Based on the hemocyte cell line SYSU-OfHe-C established at our lab, two cell sublines, SYSU-OfHe-C Granulocyte (Gr cells) and SYSU-OfHe-C Plasmatocyte (Pl cells), which possess the morphological characteristics of granulocytes and plasmatocytes, respectively, were established. Gr and Pl cells showed different behaviors in immune reactions, such as spreading, phagocytosis and encapsulation. Pl cells were easier to spread, but Gr cells tended to undergo aggregation, indicating that they may take different strategies to clear foreign objects. These results also suggested that granulocytes and plasmatocytes may express some different proteins. By comparing the gene expression in cells from the two sublines, 1662 differentially expressed genes were identified, and 13 out of 30 transmembrane proteins highly expressed in Pl cells (six) or Gr cells (seven) were further screened and confirmed by reverse-transcription polymerase chain reaction (PCR). Finally, three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis. These genes may potentially be used as molecular markers to distinguish between granulocytes and plasmatocytes in Ostrinia furnacalis, and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.

中文翻译：

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亚洲玉米螟两个连续血细胞亚系的建立及血细胞分子标记的鉴定

粒细胞和浆细胞在清除昆虫体内异物方面起重要作用，但在免疫反应中很难区分。基于我们实验室建立的血细胞系SYSU - Of He-C，两个细胞亚系SYSU - Of He-C Granulocyte (Gr cells)和SYSU- Of建立了分别具有粒细胞和浆细胞形态特征的He-C浆细胞(Pl细胞)。Gr 和 Pl 细胞在免疫反应中表现出不同的行为，如扩散、吞噬和封装。Pl细胞更容易扩散，但Gr细胞倾向于聚集，表明它们可能采取不同的策略来清除异物。这些结果还表明粒细胞和浆细胞可能表达一些不同的蛋白质。通过比较两个亚系细胞中的基因表达，鉴定出1662个差异表达基因，30个在Pl细胞（6个）或Gr细胞（7个）中高表达的跨膜蛋白中的13个通过逆转录聚合酶进一步筛选确认链式反应 (PCR)。最后，基于免疫反应中的表达，通过定量PCR分析筛选出Pl细胞特异表达的3个跨膜基因和Gr细胞特异表达的2个跨膜基因。这些基因可能被用作分子标记来区分粒细胞和浆细胞Ostrinia furnacalis，进一步阐明免疫血细胞在细胞免疫反应中的作用，如包囊等。