Abstract

The apple is a highly perishable fruit after harvesting and, therefore, several storage technologies have been studied to provide the consumer market with a quality product with a longer shelf life. However, little is known about the apple genome that is submitted to the storage, and even less with the application of ripening inhibitors. Due to these factors, this study sought to elucidate the transcriptional profile of apple cultivate Gala stored in a controlled atmosphere (AC) treated and not treated with 1-methyl cyclopropene (1-MCP). Through the genetic mapping of the apple, applying the microarray technique, it was possible to verify the action of treatments on transcripts related to photosynthesis, carbohydrate metabolism, response to hormonal stimuli, nucleic acid metabolism, reduction of oxidation, regulation of transcription and metabolism of cell wall and lipids. The results showed that the transcriptional profile in the entire genome of the fruit showed significant differences in the relative expression of the gene, this in response to CA in the presence and absence of 1-MCP. It should be noted that the transcription genes involved in the anabolic pathway were only maintained after six months in fruits treated with 1-MCP. The data in this work suggests that the apple in the absence of 1-MCP begins to prepare its metabolism to mature, even during the storage period in AC. Meanwhile, in the presence of the inhibitor, the transcriptional profile of the fruit is similar to that at the time of harvest. It was also found that a set of genes that code for ethylene receptors, auxin homeostasis, MADS Box, and NAC transcription factors may be involved in the regulation of post-harvest ripening after storage and in the absence of 1-MCP.

Graphic abstract

中文翻译：

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1-MCP在分子水平上对受控气氛苹果果实的技术优势。

摘要

苹果是收获后极易腐烂的水果，因此，已研究了多种存储技术，以为消费市场提供具有较长保质期的优质产品。但是，对于提交给储存库的苹果基因组知之甚少，而对于成熟抑制剂的应用则知之甚少。由于这些因素，本研究试图阐明存储在受控气氛（AC）中的苹果栽培晚会的转录谱，该受控气氛未经过1-甲基环丙烯（1-MCP）处理。通过苹果的遗传图谱，应用微阵列技术，可以验证治疗对与光合作用，碳水化合物代谢，对激素刺激的反应，核酸代谢，氧化减少，调节细胞壁和脂质的转录和代谢。结果表明，在整个水果基因组中的转录谱显示该基因的相对表达存在显着差异，这是在存在和不存在1-MCP的情况下对CA的响应。应该注意的是，参与合成代谢途径的转录基因仅在六个月后在1-MCP处理的果实中得以维持。这项工作中的数据表明，即使没有AC储存，在没有1-MCP的情况下，苹果也开始使其新陈代谢成熟。同时，在存在抑制剂的情况下，果实的转录特征与收获时的相似。还发现了一组编码乙烯受体，植物生长素稳态，MADS Box，

图形摘要