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Bioinformatical dissection of fission yeast DNA replication origins.
Open Biology ( IF 5.8 ) Pub Date : 2020-07-22 , DOI: 10.1098/rsob.200052
Koji Masuda 1 , Claire Renard-Guillet 1 , Katsuhiko Shirahige 1 , Takashi Sutani 1
Affiliation  

Replication origins in eukaryotes form a base for assembly of the pre-replication complex (pre-RC), thereby serving as an initiation site of DNA replication. Characteristics of replication origin vary among species. In fission yeast Schizosaccharomyces pombe, DNA of high AT content is a distinct feature of replication origins; however, it remains to be understood what the general molecular architecture of fission yeast origin is. Here, we performed ChIP-seq mapping of Orc4 and Mcm2, two representative components of the pre-RC, and described the characteristics of their binding sites. The analysis revealed that fission yeast efficient origins are associated with two similar but independent features: a ≥15 bp-long motif with stretches of As and an AT-rich region of a few hundred bp. The A-rich motif was correlated with chromosomal binding of Orc, a DNA-binding component in the pre-RC, whereas the AT-rich region was associated with efficient binding of the DNA replicative helicase Mcm. These two features, in combination with the third feature, a transcription-poor region of approximately 1 kb, enabled to distinguish efficient replication origins from the rest of chromosome arms with high accuracy. This study, hence, provides a model that describes how multiple functional elements specify DNA replication origins in fission yeast genome.



中文翻译:

裂殖酵母 DNA 复制起点的生物信息学解剖。

真核生物中的复制起点形成了组装前复制复合体 (pre-RC) 的基础,从而充当 DNA 复制的起始位点。复制起点的特征因物种而异。在裂变酵母中粟酒裂殖酵母,高AT含量的DNA是复制起点的显着特征;然而,裂殖酵母起源的一般分子结构是什么还有待了解。在这里,我们对 Pre-RC 的两个代表性组件 Orc4 和 Mcm2 进行了 ChIP-seq 映射,并描述了它们结合位点的特征。分析表明,裂殖酵母的高​​效起源与两个相似但独立的特征相关:一个≥15 bp 长的基序,带有一段 As 和一个几百 bp 的富含 AT 的区域。富含 A 的基序与 Orc(前 RC 中的 DNA 结合成分)的染色体结合相关,而富含 AT 的区域与 DNA 复制解旋酶 Mcm 的有效结合相关。这两个特征,结合第三个特征,大约 1 kb 的转录贫乏区域,能够以高精度区分有效的复制起点和其余的染色体臂。因此,这项研究提供了一个模型,该模型描述了多个功能元件如何指定裂殖酵母基因组中的 DNA 复制起点。

更新日期:2020-07-22
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