Herein, we have designed and synthesized new imidazo[2,1-b]thiazole-based aryl hydrazones (9a–w) and evaluated their anti-proliferative potential against a panel of human cancer cell lines. Among the synthesized compounds, 9i and 9m elicited promising cytotoxicity against the breast cancer cell line MDA-MB-231 with IC₅₀ values of 1.65 and 1.12 μM, respectively. Cell cycle analysis revealed that 9i and 9m significantly arrest MDA-MB-231 cells in the G0/G1 phase. In addition, detailed biological studies such as annexin V-FITC/propidium iodide, DCFH-DA, JC-1 and DAPI staining assays revealed that 9i and 9m triggered apoptosis in MDA-MB-213 cells. Overall, the current work demonstrated the cytotoxicity and apoptosis-inducing potential of 9i and 9m in breast cancer cells and suggested that they could be explored as promising antiproliferative leads in the future.

中文翻译：

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新的咪唑并[2,1-b]噻唑基芳基：揭示它们的合成及其抗增殖和诱导凋亡的潜力

在这里，我们设计并合成了新的基于咪唑并[2,1 - b ]噻唑的芳基（9a–w），并评估了它们对一组人类癌细胞系的抗增殖潜力。在合成的化合物中，9i和9m引发了针对乳腺癌细胞系MDA-MB-231的有希望的细胞毒性，IC ₅₀值分别为1.65和1.12μM。细胞周期分析显示9i和9m将MDA-MB-231细胞显着阻滞在G0 / G1期。此外，详细的生物学研究（例如膜联蛋白V-FITC /碘化丙啶，DCFH-DA，JC-1和DAPI染色试验）显示9i和9m触发了MDA-MB-213细胞的凋亡。总的来说，目前的研究证明了9i和9m在乳腺癌细胞中具有细胞毒性和诱导细胞凋亡的潜力，并建议将来可以将它们作为有希望的抗增殖药加以研究。