Steroid-induced avascular necrosis of the femoral head (SANFH) is a major complication of long-term or excessive clinical use of glucocorticoids. Allicin is a classical ingredient extracted from garlic and has many functions such as anti-apoptosis and antibacterial effects. The purpose of this study was to investigate the effect and the mechanism of allicin on apoptosis of osteoblasts induced by dexamethasone (Dex) and SANFH in rats. In vitro, we performed CCK-8, western blotting, TUNEL and other experiments, and the results of these experiments showed that allicin could inhibit the Dex-induced abnormal expression of C-caspase3, C-caspase9, Bax, cytochrome C and Bcl-2 by activating the PI3K/AKT pathway. In vivo, the results of micro-CT, hematoxylin–eosin staining and immunohistochemical analysis suggested that allicin could effectively inhibit the progress of SANFH in rats. In summary, our experiments indicate that allicin is a potential drug for the treatment of SANFH.

中文翻译：

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大蒜素通过激活PI3K / AKT途径抑制成骨细胞凋亡和激素诱导的股骨头坏死。

激素引起的股骨头缺血性坏死（SANFH）是长期或过度临床使用糖皮质激素的主要并发症。大蒜素是从大蒜中提取的经典成分，具有许多功能，例如抗凋亡和抗菌作用。本研究的目的是探讨大蒜素对地塞米松（Dex）和SANFH诱导的成骨细胞凋亡的影响及其机制。在体外，我们进行了CCK-8，western blotting，TUNEL等实验，这些实验结果表明大蒜素可以抑制Dex诱导的C-caspase3，C-caspase9，Bax，细胞色素C和Bcl-的异常表达。 2通过激活PI3K / AKT途径。体内显微CT，苏木精-伊红染色和免疫组化分析结果表明，大蒜素可以有效抑制大鼠SANFH的进程。总而言之，我们的实验表明大蒜素是用于治疗SANFH的潜在药物。