N ^ε-(Carboxymethyl)lysine (CML), which is formed by the glycation of collagen, is a skin-accumulating advanced glycation end product and has been shown to be deeply involved in diabetic osteopenia and skin aging. In this study, we prepared the phlorotannins of marine algal polyphenols from Japanese Lessoniaceae (Ecklonia cava, Ecklonia kurome, cultured E. kurome, Ecklonia stolonifera, Eisenia nipponica, and Eisenia bicyclis) and evaluated their inhibitory activities against CML formation in a collagen-glyoxal environment. The level of CML formed from the glycation of collagen by glyoxal was detected using an enzyme-linked immunosorbent assay. Except for E. stolonifera, the level of CML formation in the treatment with crude phlorotannins at 0.16 µg/mL was found to be comparable to that in the treatment with 0.40 mM aminoguanidine hydrochloride (AG) which is a typical antiglycation agent. In the test using phloroglucinol and isolated eckols (eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, and 8,8’-bieckol) at a concentration of 0.80 µg/mL, the level of CML formed was lower for each compound, except for phlorofucofuroeckol A, than the data obtained with the addition of 2.0 mM AG. The mass concentration of 0.80 µg/mL was converted to 6.3 µM for phloroglucinol, 2.2 µM for eckol, 1.7 µM for fucofuroeckol A, 1.3 µM for phlorofucofuroeckol A, and 1.1 µM for dieckol and 8,8’-bieckol. From a comparison of the molar concentrations, it was found that phloroglucinol and the eckols inhibited the formation of CML resulting from glycation of collagen by glyoxal at concentrations of approximately 317 to 1818 times lower than AG.

Ñ ^ε - （羧甲基）赖氨酸（CML），这是由胶原蛋白的糖化形成的，是一种皮肤累积糖化终产物，并已被证明是深度参与糖尿病骨质减少和皮肤老化。在这项研究中，我们制备了日本柏木科（Ecklonia cava，Ecklonia kurome，培养的E. kurome，Ecklonia stolonifera，Eisenia nipponica和Eisenia bicyclis）海洋藻类多酚的叶绿素，并评估了它们对胶原-乙二醛中CML形成的抑制活性。环境。使用酶联免疫吸附测定法检测由乙二醛由胶原糖基化形成的CML水平。除了stolonifera，发现以0.16 µg / mL的粗环戊单宁处理的CML形成水平与典型的抗糖化剂0.40 mM氨基胍盐酸盐（AG）的处理水平相当。在使用间苯三酚和分离的eckols（eckol，fucofuroeckol A，phlorofucofuroeckol A，dieckol和8,8'-bieckol）以0.80 µg / mL的浓度进行的测试中，每种化合物的CML形成水平都较低，除了phofofucofuroeckolol A，而不是添加2.0 mM AG所获得的数据。将质量浓度为0.80 µg / mL的间苯三酚转换为6.3 µM，对eckol转换为2.2 µM，对氟呋喃酚A调整为1.7 µM，对呋喃氟酚A调整为1.3 µM，对二萘酚和8,8'-bieckol转换为1.1 µM。通过摩尔浓度的比较，