Abstract

This paper describes a transcriptomic profiling of Paracoccidioides brasiliensis (Pb) performed with the aid of an RNA-seq-based approach, aimed at characterizing the general transcriptome in this human pathogenic fungus, responsible for paracoccidioidomycosis (PCM). Results confirm that ∼75% of the genes currently annotated in the P. brasiliensis genome are, in fact, transcribed in vivo and that ∼19% of them may display alternative isomorphs. Moreover, we identified 627 transcripts that do not match any gene currently mapped in the genome, represented by 114 coding transcripts (probably derived from previously unmapped protein-coding genes) and 513 noncoding RNAs (ncRNAs), including 203 long-noncoding RNAs (lncRNAs).

中文翻译：

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转录组学分析鉴定了巴西副球孢子虫中的新转录本、同构型和非编码 RNA

摘要

本文描述了在基于 RNA 序列的方法的帮助下对巴西副球孢子菌(Pb) 进行的转录组学分析，旨在表征这种人类致病真菌中的一般转录组，该真菌负责副球孢子菌病 (PCM)。结果证实，目前在P 中注释的基因约有 75% 。brasiliensis基因组实际上是在体内转录的，其中约 19% 可能显示出替代同形体。此外，我们鉴定了 627 个与基因组中当前定位的任何基因都不匹配的转录本，由 114 个编码转录本（可能来自之前未定位的蛋白质编码基因）和 513 个非编码 RNA（ncRNA）代表，其中包括 203 个长链非编码 RNA（lncRNA） ）。