ABSTRACT

Objective: Assess the variability and differences in oxidative stress, antioxidant, and inflammatory biomarkers in people with type 2 diabetes mellitus (T2D) and healthy controls.

Methods:: Ten men and women diagnosed with T2D and ten healthy matched controls (CON) were recruited. Participants had venous blood taken at six different time points on different days, three in the morning (after overnight fast) and three in the afternoon. Inflammation (IL-6, 8, 10 and TNF-α), oxidative stress/antioxidant biomarkers (F₂-isoprostanes, protein carbonyls, total antioxidant capacity (TAC), glutathione peroxidase activity, IL-6, 8 & 10 and TNF-α) were assessed.

Results:: Biomarker concentrations were similar between groups. There was large variability in nearly all biomarkers for both groups. For inflammatory measures, intra-individual coefficients of variation (CV) ranged from 64.0–92.1% and 100.9–259.0% for inter-individual differences. CVs for oxidative stress markers were lower (7.4–31.2% for intra-individual and 8.6–43.0% for inter-individual). TAC had the lowest intra-individual CV – 7% for T2D and 8% for CON. Protein carbonyls were more variable in the afternoon (34% CV) compared to morning (24% CV) in CON. IL-6 intra-individual CV was different between groups for afternoon measurements (93% T2D, 60% CON).

Conclusion:: Oxidative stress and inflammatory biomarkers show considerable variation in both T2D and healthy populations.

Trial registration: ClinicalTrials.gov identifier: NCT01206725.

摘要

目的：评估2型糖尿病（T2D）患者和健康对照者的氧化应激，抗氧化剂和炎性生物标志物的变异性和差异。

方法：招募十名被诊断患有T2D的男性和十名健康匹配对照（CON）。参与者在不同日期的六个不同时间点采集了静脉血，早上三点（禁食过夜后）和下午三点。炎症（IL- 6、8、10和TNF-α），氧化应激/抗氧化剂生物标志物（F _2-异前列腺素，蛋白羰基，总抗氧化能力（TAC），谷胱甘肽过氧化物酶活性，IL-6、8和10和TNF- α）被评估。

结果：组之间的生物标志物浓度相似。两组的几乎所有生物标志物均存在较大差异。对于炎症措施，个体间差异的个体内变异系数（CV）为64.0–92.1％和100.9–259.0％。氧化应激指标的CV值较低（个体内部为7.4–31.2％，个体间为8.6–43.0％）。TAC的个人内部CV最低-T2D为7％，CON为8％。与早晨（24％CV）相比，下午的CON中蛋白羰基的变化更大（CV为34％）。下午测量的两组间IL-6个体内CV有所不同（93％T2D，60％CON）。

结论：氧化应激和炎性生物标志物在T2D和健康人群中均表现出相当大的差异。

试用注册： ClinicalTrials.gov标识符：NCT01206725。