Abstract

The objective of this study was to construct a microbial consortium for effective biodegradation of chlorpyrifos (CP) and its hydrolysis product, 3,5,6-Trichloro-2-pyridinol (TCP). A activated sludge from an organophosphorus pesticide factory was domesticated under long-term (20 weeks) CP stress, and the dynamic change in bacterial communities was analyzed by high-throughput sequencing. Then, a microbial consortium MC-BSPK was constructed of Bacillus sp. MC-B, Serratia sp. MC-S, Pseudomonas sp. MC-P, and Klebsiella sp. MC-K, which were significantly enriched during the domestication process. The biodegradation capacities of the microbial consortium MC-BSPK reached 100% for CP within 9 days and 88.61% for TCP within 15 days under the optimized degradation conditions (pH 8.0 and 31 °C). High-performance liquid chromatography (HPLC) revealed that CP could be degraded by the microbial consortium MC-BSPK into TCP, probably through hydrolysis of the P–O ester bond, and further degraded into other small molecules. A bioassay revealed that the virulence of CP toward Drosophila melanogaster W1118 was clearly reduced by the microbial consortium MC-BSPK biodegradation. Thus, the easily constructed microbial consortium MC-BSPK with high CP/TCP degradation capacities has the potential for application in pesticide-contaminated bioremediation.

摘要

这项研究的目的是建立一个微生物财团，以有效地降解毒死rif（CP）及其水解产物3,5,6-三氯-2-吡啶醇（TCP）。在长期（20周）CP胁迫下驯化了有机磷农药工厂的活性污泥，并通过高通量测序分析了细菌群落的动态变化。然后，由芽孢杆菌属（Bacillus sp。）构建了微生物联盟MC-BSPK 。MC-B，沙雷氏菌。MC-S，假单胞菌属。MC-P和克雷伯氏菌sp。MC-K，在驯化过程中显着富集。在优化的降解条件（pH 8.0和31°C）下，微生物财团MC-BSPK在9天内对CP的生物降解能力达到100％，在15天内对TCP的生物降解能力达到88.61％。高效液相色谱法（HPLC）表明，微生物财团MC-BSPK可能会将CP降解为CP，可能是通过P–O酯键的水解，然后进一步降解为其他小分子。生物测定表明，CP对果蝇具有毒力。微生物协会MC-BSPK的生物降解明显降低了W1118。因此，具有高CP / TCP降解能力的易于构建的微生物联盟MC-BSPK具有在农药污染的生物修复中应用的潜力。