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Safety and biological activity evaluation of Uncaria rhynchophylla ethanolic extract
Drug and Chemical Toxicology ( IF 2.6 ) Pub Date : 2020-07-21 , DOI: 10.1080/01480545.2020.1786581
Heung Bin Lim 1 , Hyeong Ryeol Lee 1
Affiliation  

Abstract

Uncaria rhynchophylla (UR) belongs to the Rubiaceae family, and its dried hooks are usually used in traditional medicine. It is effective in treating diseases related to the central nervous system. This study aimed to evaluate the safety of UR extract, investigate its antimutagenic and antioxidative activities, and elucidate its active components. Extraction and fractionation of the UR extract resulted in yields of 6.71%, 0.037%, 0.042%, 0.152%, 0.332%, and 5.132%, for hexane, ether, DCM, EtOAC, and aqueous fractions, respectively. The four indole alkaloids, total phenolic content (TPC), and total flavonoid content (TFC) of UR extract and its subfractions were measured. Alkaloid content was highest in the UR extract. TPC was the highest in the EtOAC fraction (373.7 ± 20.9 mg gallic acid equivalent (GAE)/g), whereas TFC was the highest in the UR extract (33.5 ± 2.4 mg quercetin equivalent (QE)/g). To assess the safety of UR extract mutagenicity, cytotoxicity, and oxidative stress inducibility assays were performed. The UR extract (2000 µg/plate) showed excellent antimutagenic activity (above 90%) against BaP in both TA98 and TA100 strains. The UR extract exhibited efficient DPPH (RC50 239.2 ± 16.5 µg/mL) and ABTS scavenging activity (RC50 458.7 ± 25.0 µg/mL). The UR extract (150 µg/mL) showed cytoprotective activity (65.6% ± 9.2%) against t-BHP. Among the subfractions, the EtOAC fraction possessed the strongest activities, overall. UR generally showed excellent biological activity at nontoxic concentrations (determined in vitro in current work), although the chemical composition of UR requires further investigation prior to its potential future use.



中文翻译:

钩藤乙醇提取物的安全性和生物活性评价

摘要

钩藤(UR)属于茜草科,其干钩通常用于传统医学。对治疗中枢神经系统相关疾病有效。本研究旨在评估乌尔提取物的安全性,研究其抗诱变和抗氧化活性,并阐明其活性成分。UR 提取物的萃取和分级分离导致己烷、乙醚、DCM、EtOAC 和水性馏分的产率分别为 6.71%、0.037%、0.042%、0.152%、0.332% 和 5.132%。测定了UR提取物及其亚组分的四种吲哚生物碱、总酚含量(TPC)和总黄酮含量(TFC)。UR提取物中生物碱含量最高。TPC 在 EtOAC 部分中最高(373.7 ± 20.9 mg 没食子酸当量 (GAE)/g),而 TFC 在 UR 提取物中最高(33.5 ± 2. 4 mg 槲皮素当量 (QE)/g)。为了评估 UR 提取物的致突变性、细胞毒性和氧化应激诱导试验的安全性。UR 提取物(2000 µg/板)对 TA98 和 TA100 菌株中的 BaP 均表现出优异的抗诱变活性(90% 以上)。UR 提取物表现出高效的 DPPH (RC50 239.2 ± 16.5 µg/mL) 和 ABTS 清除活性 (RC 50 458.7 ± 25.0 µg/mL)。UR 提取物 (150 µg/mL) 显示出对t -BHP 的细胞保护活性 (65.6% ± 9.2%)。在亚组分中,EtOAC 组分总体上具有最强的活性。UR 通常在无毒浓度下表现出优异的生物活性(在当前工作中在体外测定),尽管 UR 的化学成分在其潜在的未来用途之前需要进一步研究。

更新日期:2020-07-21
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