ABSTRACT

The apparent amylose content (AAC) is usually measured by an iodine colorimetric method using the starch as a sample. Nevertheless, it is time-consuming and labor-intensive to prepare rice starch. Therefore, we compared two methods, starch method and milled rice flour method. The former AACs were higher than the latter and correlated with pasting properties, physical properties and sensory scores better than the latter. Therefore, we developed a novel formula for estimating the AACs of starches from japonica rice cultivars using milled rice flours instead of using rice starch. The correlation coefficients of the new formula were 0.89 for calibration and 0.94 for validation, which showed that this equation can be applied to unknown japonica rice samples, and will lead to easy, rapid, and accurate iodine method to determine rice AACs using milled rice flours instead of starch.

Abbreviations: AAC: apparent amylose content; RS: resistant starch; SLC: super-long chain; CD: chain length distribution; RVA: Rapid Visco Analyzer; SB: setback; BD: breakdown; Max.vis: maximum viscosity; Min.vis: minimum viscosity; Pt: pasting temperature; Cons: consistency; Final.vis: Final viscosity; SB/Cons: setback/consistency; Max/Min: maximum viscosity/minimum viscosity; Max/Fin: maximum viscosity/final viscosity.

摘要

表观直链淀粉含量（AAC）通常通过使用淀粉作为样品的碘比色法进行测量。然而，制备米淀粉是既费时又费力的。因此，我们比较了两种方法，淀粉法和米粉法。前者的AAC高于后者，并且与后者的粘贴特性，物理特性和感官评分相关性更好。因此，我们开发了一种新的公式，用于估计使用碾米粉代替大米淀粉的粳稻品种的淀粉的AAC 。新公式的校正系数为0.89，验证系数为0.94，表明该方程式可应用于未知粳稻。 大米样品，这将导致简便，快速和准确的碘方法使用碾米粉代替淀粉来测定大米AAC。

缩写： AAC：表观直链淀粉含量；RS：抗性淀粉；SLC：超长链；CD：链长分布；RVA：快速粘度分析仪；SB：挫折；BD：故障；最大粘度：最大粘度；最小粘度：最小粘度；Pt：粘贴温度；缺点：一致性；最终粘度：最终粘度；SB /缺点：挫折/一致性；最大/最小：最大粘度/最小粘度；Max / Fin：最大粘度/最终粘度。