Identification and evaluation of long‐term markers is crucial in prolonging the detection window for anabolic steroid abuse in sport. Recently, sulfoconjugated epiandrosterone was identified as a potential long‐term marker for the abuse of certain endogenous anabolic agents, including testosterone, which continues to be widely used as a performance enhancing agent in sport. To evaluate the applicability of epiandrosterone sulfate as a marker for testosterone use, administration studies were conducted with multiple modes of testosterone administration – transdermal, intramuscular, and subcutaneous. A modified sample preparation method was used to collect both glucuronidated and sulfoconjugated analytes of interest. Carbon isotope ratio measurements from the administration studies are presented here. Epiandrosterone was less effective than the conventionally used target compounds for detection of the low dose application (transdermal gel). With intramuscular administration, epiandrosterone was more diagnostic than with transdermal administration, but it did not prolong the detection window more than the conventional target compounds. With subcutaneous administration, the doses administered to the subjects were varied and the effect on the epiandrosterone values was dependent on the magnitude of the dose administered. Epiandrosterone does not appear to be a useful marker in the detection of low dose testosterone administration. It is responsive to higher dose administration, but it does not provide an extension of the detection window relative to conventional target compounds.

中文翻译：

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表雄酮作为睾酮使用的长期标志物的评估。

长期标志物的鉴定和评估对于延长运动中合成代谢类固醇滥用的检测窗口至关重要。最近，磺基共轭表雄酮被确定为滥用某些内源性合成代谢药物（包括睾酮）的潜在长期标志物，睾酮继续被广泛用作运动中的表现增强剂。为了评估硫酸表雄酮作为睾酮使用标志物的适用性，对多种睾酮给药模式进行了给药研究——透皮、肌内和皮下。改进的样品制备方法用于收集目标分析物的葡糖醛酸化和磺基共轭分析物。此处介绍了来自管理研究的碳同位素比率测量值。表雄酮在检测低剂量应用（透皮凝胶）方面不如常规使用的目标化合物有效。肌内给药，表雄酮比透皮给药更具诊断性，但与常规目标化合物相比，它不会延长检测窗口。对于皮下给药，对受试者给药的剂量是变化的，对表雄酮值的影响取决于给药剂量的大小。表雄酮似乎不是检测低剂量睾酮给药的有用标志物。它对更高剂量的给药有反应，但它不提供相对于常规目标化合物的检测窗口的扩展。