In F. graminearum, the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15‐acetyl deoxynivalenol. Secondary metabolites are not essential for survival, instead, they enable the pathogen to successfully infect its host. F. graminearum has the potential to produce a diverse array of secondary metabolites (SMs). However, given high functional specificity and energetic cost, most of these clusters remain silent, unless the organism is subjected to an environment conducive to SM production. Alternatively, secondary metabolite gene clusters (SMCs) can be activated by genetically manipulating their activators or repressors. In this study, a combination of transcriptomic and metabolomics analyses with a deletion and overexpressor mutants of TRI6 was used to establish the role of TRI6 in the regulation of several BGCs in F. graminearum. Evidence for direct and indirect regulation of BGCs by TRI6 was obtained by chromatin immunoprecipitation and yeast two‐hybrid experiments. The results showed that the trichothecene genes are under direct control, while the gramillin gene cluster is indirectly controlled by TRI6 through its interaction with the pathway‐specific transcription factor GRA2.

中文翻译：

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禾本科镰刀菌中的全球转录调节因子TRI6激活生物合成基因簇。

在禾谷镰刀菌中，转录因子TRI6积极调节单端孢菌素生物合成基因簇（BGC），导致产生次生代谢产物15-乙酰基脱氧雪茄烯醇。次生代谢产物不是存活所必需的，而是使病原体成功感染其宿主。禾本科有潜力产生各种各样的次级代谢产物（SM）。但是，鉴于高功能特异性和精力充沛的成本，除非有机体处于有利于SM生产的环境下，否则大多数这些簇仍然保持沉默。或者，可通过遗传操纵其激活因子或阻遏因子来激活次级代谢产物基因簇（SMC）。在这项研究中，使用转录组学和代谢组学分析与TRI6的缺失和过表达突变体相结合，来确定TRI6在禾谷镰孢中几个BGC的调控中的作用。。通过染色质免疫沉淀和酵母双杂交实验获得了TRI6对BGC的直接和间接调控的证据。结果表明，单端孢菌烯基因受直接控制，而gramillin基因簇则通过与通路特异性转录因子GRA2的相互作用而被TRI6间接控制。