Pancreatic cancer patients are asymptomatic at early stages and leading to late diagnoses. Additionally, pancreatic cancer easily metastasizes and is resistant to radiotherapy and chemotherapy. Therefore, it is critical to understand the underlying molecular mechanisms involved in pancreatic cancer to develop more efficient diagnostic and treatment strategies. In this study, we demonstrated that circRHOT1 was overexpressed in pancreatic cancer tissues and cell lines, and it was found to directly bind to miR‐125a‐3p, acting as an endogenous sponge to inhibit its activity. Knockdown of circRHOT1 expression significantly inhibited proliferation as well as invasion, and it promoted apoptosis of pancreatic cancer cells via the regulation of E2F3 through the targeting of miR‐125a‐3p. Taken together, our results showed that circRHOT1 plays critical roles in regulating the biological functions of pancreatic cancer cells, suggesting that circRHOT1 may serve as a potential diagnostic marker and therapeutic target for patients with pancreatic cancer.

中文翻译：

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CircRHOT1通过海绵miR-125a-3p介导胰腺癌细胞的增殖、凋亡和侵袭。

胰腺癌患者早期无症状，导致诊断较晚。此外，胰腺癌容易转移并且对放疗和化疗有抵抗力。因此，了解胰腺癌的潜在分子机制以制定更有效的诊断和治疗策略至关重要。在这项研究中，我们证明了 circRHOT1 在胰腺癌组织和细胞系中过表达，并且发现它直接与 miR-125a-3p 结合，充当内源性海绵来抑制其活性。抑制circRHOT1表达可显着抑制增殖和侵袭，并通过靶向miR-125a-3p调节E2F3促进胰腺癌细胞凋亡。综合起来，