The more complex 3′ UTR in higher organisms may have the function of increasing post‐transcriptional gene regulation. Recent RNA sequencing technologies have provided us with the possibility to capture the complete 3′ UTR landscape of different species and cells. However, no systematic analysis of sheep‐related 3′ UTR has been performed. Here, we conducted a detailed analysis of the 3′ UTR with the primary goal of identifying intact 3′ UTR landscapes in the sheep muscles of the three developmental stages. Based on strand‐specific RNA sequencing (ssRNA‐seq) data, we found that thousands of genes in sheep muscle are continuously transcribed after the UTR of the reference genome (Oar_v4.0). More than 66% of the 3′ UTR extensions exhibit similar expression trends to their upstream gene exons. These 3′ UTR extensions strongly enrich thousands of conserved microRNA binding sites. The 3′ UTR extension‐associated RNA of PFKM (PuaRNA) was predicted to be derived from the 3′ UTR of PFKM. In sheep myocytes, myotubes and various tissues, the expression pattern of PuaRNA is positively correlated with that of PFKM. Taken together, these new 3′ UTR annotations greatly extend the range of mammalian post‐transcriptional regulatory networks, which have a particular impact on the regulation of sheep muscle development.

中文翻译：

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重新解释显示大量3'UTR延伸的绵羊肌肉链特异性RNA测序数据。

高等生物中较复杂的3'UTR可能具有增加转录后基因调控的功能。最新的RNA测序技术为我们提供了捕获不同物种和细胞的完整3'UTR景观的可能性。但是，尚未对绵羊相关的3'UTR进行系统分析。在这里，我们对3'UTR进行了详细分析，其主要目的是确定三个发育阶段的绵羊肌肉中完整的3'UTR景观。根据链特异性RNA测序（ssRNA-seq）数据，我们发现在参考基因组（Oar_v4.0）的UTR之后，绵羊肌肉中的数千个基因被连续转录。超过66％的3'UTR延伸片段显示出与其上游基因外显子相似的表达趋势。这些3'UTR扩展强烈丰富了数千个保守的microRNA结合位点。3'UTR延伸相关的RNAPFKM（PuaRNA）预计源自PFKM的3'UTR 。在羊肌细胞，肌管和各种组织，PuaRNA的表达模式是积极与该相关的PFKM。综上所述，这些新的3'UTR注释大大扩展了哺乳动物转录后调控网络的范围，这对绵羊肌肉发育的调控具有特殊的影响。