ABSTRACT Treatment of skin wounds is an important domain in biomedical research since many pathogenic bacteria can invade the damaged tissues causing serious infections. Effective treatments are required under such conditions to inhibit microbial growth. Plants are traditionally used for the treatment of skin infections due to their antimicrobial potential. The antibacterial activity of different solvent extracts of four Hypericum species (H. androsaemum, H. ericoides, H. x moserianum and H. olympicum) traditionally acclaimed for their wound healing activity was examined in the present study against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterobacter aerogenes. In addition the content and types of flavonoids [High Performance Liquid Chromatography (HPLC) analysis], and antioxidant activity [1,1-diphenyl-2-picryIhydrazyl (DPPH) assay] were evaluated for all the species. The most prominent antibacterial activity was displayed by H. olympicum (MIC between 0.001 – 0.1 mg/mL) in particular ethyl acetate and n-butanol fractions which were found to be rich in phenolic and flavonoid contents. Strong antioxidant activity was observed for all the species and was associated with the more polar methanol, ethyl acetate and n-butanol extracts, with IC50 values ranging between 0.093 to 0.3 mg/mL. HPLC analysis of the extracts indicated the presence of different flavonoids in the plants and the highest content of selected flavonoids was determined for H. olympicum. The antibacterial activity of the selected Hypericum species shown in this study supports the traditional role of using these species for wound healing.

中文翻译：

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英国四种金丝桃属植物的植物化学特征、抗氧化和抗菌活性

摘要皮肤伤口的治疗是生物医学研究的一个重要领域，因为许多病原菌可以侵入受损组织，导致严重感染。在这样的条件下需要有效的处理来抑制微生物生长。由于其抗菌潜力，植物传统上用于治疗皮肤感染。在本研究中，研究了传统上因其伤口愈合活性而广受赞誉的四种金丝桃属植物（H. androsaemum、H. ericoides、H. x moserianum 和 H. olympicum）的不同溶剂提取物对枯草芽孢杆菌、金黄色葡萄球菌、埃希氏菌的抗菌活性大肠杆菌、铜绿假单胞菌和产气肠杆菌。此外，黄酮类化合物的含量和类型 [高效液相色谱 (HPLC) 分析] 和抗氧化活性 [1, 对所有物种进行了 1-二苯基-2-苦味酰肼 (DPPH) 测定]。最突出的抗菌活性是由 H. olympicum（MIC 在 0.001 – 0.1 mg/mL 之间），特别是发现富含酚类和类黄酮含量的乙酸乙酯和正丁醇馏分。对所有物种都观察到了很强的抗氧化活性，并且与极性更大的甲醇、乙酸乙酯和正丁醇提取物有关，IC50 值范围在 0.093 到 0.3 mg/mL 之间。提取物的 HPLC 分析表明植物中存在不同的黄酮类化合物，并且确定了 H. olympicum 中所选黄酮类化合物的最高含量。本研究中显示的所选金丝桃属植物的抗菌活性支持使用这些物种进行伤口愈合的传统作用。