Journal of Virological Methods ( IF 3.1 ) Pub Date : 2020-07-22 , DOI: 10.1016/j.jviromet.2020.113943 Petra C Fay 1 , Charlotte G Cook 1 , Najith Wijesiriwardana 1 , Gessica Tore 1 , Loic Comtet 2 , Alix Carpentier 2 , Barbara Shih 3 , Graham Freimanis 1 , Ismar R Haga 1 , Philippa M Beard 4
Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population in these regions. An increase in research efforts into LSDV is required to address key knowledge gaps, however this is hampered by lack of suitable cell lines on which to propagate and study the virus. In this work we describe the replication and spread of LSDV on Madin-Darby bovine kidney (MDBK) cells, and the formation of foci-type poxvirus plaques by LSDV on MDBK cells. Methods utilising MDBK cells to quantify neutralising antibodies to LSDV, and to purify LSDV genomic DNA suitable for short read sequencing are described. These research methods broaden the tools available for LSDV researchers and will facilitate the gathering of evidence to underpin the development of LSD control and prevention programmes.
中文翻译:
Madin-Darby牛肾(MDBK)细胞是用于牛痘病毒块状皮肤病病毒的繁殖和研究的合适细胞系。
块状皮肤病病毒(LSDV)是一种痘病毒,会引起牛的全身性疾病,给受影响的社区造成重大经济损失。LSDV是迅速引起全球关注的病原体,最近从非洲和中东传播到欧洲和亚洲,影响了这些地区的牛群。为了解决关键的知识空白,需要加大对LSDV的研究力度,但是由于缺乏在其上传播和研究病毒的合适细胞系而受到阻碍。在这项工作中,我们描述了LSDV在Madin-Darby牛肾(MDBK)细胞上的复制和传播,以及LSDV在MDBK细胞上形成病灶型痘病毒斑块。描述了利用MDBK细胞定量针对LSDV的中和抗体并纯化适于短读测序的LSDV基因组DNA的方法。