Pinus thunbergii is a black pine that has been widely used in health-promoting foods. This study was conducted to investigate the hepatoprotective effects of the alcohol-aqueous extract of Pinus thunbergii PARL leaves (PTE) in ethanol-induced liver-damaged mice. PTE increased the weight and survival rate of mice while reducing the levels of serum hepatic marker enzymes. PTE also restored the levels of antioxidant enzymes in liver tissues while drastically increasing lipid peroxidation concentrations. Histopathological examination of PTE-treated mice presented relatively less lipid droplets and hepatic tissue damage compared to ethanol-treated mice. Finally, to identify PTE components, Natural Products Application Solution with UNIFI along with an in-house library was processed and results revealed 35 compounds in the extract. These compounds were reported to exhibit significant antioxidant and/or hepatoprotective properties. Our results suggest that PTE improves liver function and can be developed as a food product with hepatoprotective effects.

黑松（Pinus thunbergii）是一种黑松，已广泛用于促进健康的食品。进行了这项研究以研究黑松（Pinus thunbergii）的醇水提取物对肝脏的保护作用。乙醇诱导的肝损伤小鼠的PARL叶（PTE）。PTE增加了小鼠的体重和存活率，同时降低了血清肝标志物酶的水平。PTE还恢复了肝脏组织中抗氧化酶的水平，同时大大增加了脂质过氧化的浓度。与乙醇处理的小鼠相比，PTE处理的小鼠的组织病理学检查显示相对较少的脂质滴和肝组织损伤。最后，为鉴定PTE成分，对带有UNIFI的Natural Products Application Solution和一个内部库进行了处理，结果显示提取物中含有35种化合物。据报道这些化合物显示出显着的抗氧化剂和/或肝保护特性。我们的结果表明，PTE可以改善肝功能，可以开发为具有保肝作用的食品。