A hybrid micelle based mobile phase was used to develop and validate a liquid chromatographic method for the separation and quantification of two local anesthetics namely; lidocaine hydrochloride (LID), and bupivacaine hydrochloride (BPV) in presence of the frequently co administered vasopressors phenyl ephrine (PHR) and ephedrine (EPH). Optimization of chromatographic separation conditions was performed applying experimental one factor at a time tool, and design of experiment, where the retention behavior of all analytes using both optimization protocols was in accordance. Chromatographic separation was carried on a C8 column operating at 40°C at a flow rate of 1.5 mL/min. using a mobile phase consisting of 0.18 M sodium dodecyl sulphate, 10% acetonitrile, containing 0.3% triethyl amine and adjusted to pH 7 using 2M ortho phosphoric acid, adopting UV detection at 230 nm. The proposed method was fully validated and applied to both in vitro and in vivo analysis of rat blood samples. The pharmacokinetics of both LID and BPV was followed when they were solitary injected or when co administered with either PHR or EPH. Moreover, the in vitro spiked experiment was also subjected to documented bio-analytical validation procedures.

基于混合胶束的流动相用于开发和验证一种液相色谱方法，用于分离和定量两种局部麻醉剂：盐酸利多卡因（LID）和盐酸布比卡因（BPV）在经常同时使用血管加压药苯肾上腺素（PHR）和麻黄碱（EPH）的情况下。一次应用实验一个因素进行色谱分离条件的优化，并设计实验，其中使用两种优化方案对所有分析物的保留行为均符合要求。在40℃下以1.5mL / min的流速在C8柱上进行色谱分离。使用由0.18 M的十二烷基硫酸钠，10％的乙腈，0.3％的三乙胺组成的流动相，并使用2M的正磷酸调节至pH 7，采用230 nm的紫外线检测。所提出的方法已得到充分验证，并应用于两者大鼠血样的体外和体内分析。LID和BPV单独注射时或与PHR或EPH共同给药时，均遵循LID和BPV的药代动力学。此外，体外加标实验也要进行文件化的生物分析验证程序。