It is widely recognized that quality of spermatozoa in sexed semen (SS) samples is not as great as for conventional, non-sexed semen (NS). There are differences in qualitative and biochemical variables between spermatozoa in NS and SS. Information, however, is lacking on molecular differences, especially concerning spermatozoa proteomic differences is SS and NS. The objective of this study was to compare commercially available NS and SS bull semen by evaluating sperm quality variables in conjunction with a proteomics approach. Results from flow cytometry and computer-assisted sperm analyses indicated there was less sperm motility, viability, mitochondrial potential and acrosome integrity in sperm from SS. Results from proteomic analysis indicated sperm from NS and SS samples were characterized by different protein profiles. There was identification of 70 sperm proteins that differed in abundance and six protein spots that were different in extent of carbonylation. Sperm from SS had altered structures of enzymes involved in glycolysis, oxidative phosphorylation and maintenance of a constant adenylate energy charge. Furthermore, sperm from SS had alterations of several flagella substructures, especially outer dense fiber proteins, which were less abundant and more carbonylated than in sperm from NS. In sperm of SS, compared with NS, there were differences in abundance of proteins involved in capacitation, acrosome reaction and sperm-egg fusion as well as lesser abundances of sperm surface proteins. Results enable a greater understanding of differences between sperm from NS and SS samples, thereby contributing to development of improved protocols for more effective protection of sexed spermatozoa during processing.

人们普遍认为，性控精液 (SS) 样本中的精子质量不如传统的非性控精液 (NS)。NS 和 SS 的精子在定性和生化变量上存在差异。然而，缺乏关于分子差异的信息，特别是关于精子蛋白质组学差异的信息是 SS 和 NS。本研究的目的是通过结合蛋白质组学方法评估精子质量变量来比较市售 NS 和 SS 公牛精液。流式细胞术和计算机辅助精子分析的结果表明来自 SS 的精子的精子活力、活力、线粒体潜力和顶体完整性较低。蛋白质组学分析的结果表明来自 NS 和 SS 样本的精子具有不同的蛋白质谱。鉴定了 70 种丰度不同的精子蛋白质和 6 种羰基化程度不同的蛋白质点。来自 SS 的精子改变了参与糖酵解、氧化磷酸化和维持恒定腺苷酸能量电荷的酶的结构。此外，来自 SS 的精子的几个鞭毛亚结构发生了改变，尤其是外部致密的纤维蛋白，与来自 NS 的精子相比，它们的含量更少且羰基化程度更高。与NS相比，SS精子中参与获能、顶体反应和精卵融合的蛋白质丰度存在差异，精子表面蛋白丰度较低。结果有助于更好地了解来自 NS 和 SS 样本的精子之间的差异，