A total of 2561 gamma ray-induced mutants (M₇) were evaluated in six abiotic stress treatments, and then principal component analysis was performed to score their phenotypes. Resequencing was performed on 123 selected rice mutants and wild-type rice to assess the structural changes in the genomes of these mutants, such as single nucleotide polymorphisms (SNPs) and insertion/deletion (InDels). A total of 5160 (avg. 87.3 per plant) SNPs and 7195 (avg. 121.9) InDels were observed in the 200-Gy lines, whereas 5650 (avg. 88.3) SNPs and 7676 (avg. 119.9) InDels were found in the 300-Gy lines. We found that one candidate gene (Os02g0528900, which is similar to the gene for pleiotropic drug resistance (PDR)-like ATP-binding cassette (ABC) transporter) contained two SNPs that were included in principal component 4 via a genome-wide association study. The 300-42 line had the lowest phenotype score against heat, possibly suggesting the existence of a relationship between these SNPs and heat stress. Co-expression analysis of the candidate gene involved in responses to toxic substances and the chemical stimulation of significant biological processes. Therefore, the studied targeting induced local lesions in genome (TILLING) population could be a useful source of mutations in the breeding of new genetic resources for forward and reverse genetics.

在六个非生物胁迫处理中评估了总共2561个伽马射线诱导的突变体（M ₇），然后进行主成分分析以评分其表型。对123个选定的水稻突变体和野生型水稻进行了重测序，以评估这些突变体的基因组结构变化，例如单核苷酸多态性（SNP）和插入/缺失（InDels）。在200-Gy品系中共观察到5160个（平均每株植物87.3个）InDel和7195个（平均121.9）InDel，而在300个品系中发现了5650个（平均88.3）SNP和7676个（平均119.9）InDel。 -Gy线。我们发现了一个候选基因（Os02g0528900（类似于多效性耐药性（PDR）样ATP结合盒（ABC）转运蛋白的基因），包含两个SNP，通过全基因组关联研究将其包含在主要成分4中。300-42系的抗热表型得分最低，这可能表明这些SNP与热应激之间存在关系。候选基因共表达分析，涉及对有毒物质的响应和重要生物过程的化学刺激。因此，针对正向和反向遗传学的新遗传资源育种中，针对靶向基因组局部诱变（TILLING）的研究可能是有用的突变来源。