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Effects of the carbon source on the physiology and invertase activity of the yeast Saccharomyces cerevisiae FT858.
3 Biotech ( IF 2.8 ) Pub Date : 2020-07-22 , DOI: 10.1007/s13205-020-02335-w
Valkirea Matos Nascimento 1 , Gabriela Totino Ulian Antoniolli 2 , Rodrigo Simões Ribeiro Leite 2 , Gustavo Graciano Fonseca 1
Affiliation  

Saccharomyces cerevisiae FT858 is an industrial yeast strain with high fermentative efficiency, but marginally studied so far. The aim of this work was to evaluate the biotechnological potential of S. cerevisiae FT858 through kinetic growth parameters, and the influence of the concentration of the substrate on the synthesis of the invertase enzyme. Invertases have a high biotechnological potential and their production through yeast is strongly influenced by the sugars in the medium. S. cerevisiae FT858 has an excellent biotechnological potential compared to the industrial yeast reference S. cerevisiae CAT-1, as it presented a low glycerol yield on the substrate (YGLY/S) and a 10% increase in ethanol yield on sucrose in cultures with sucrose at 37 °C. The substrate concentration directly interfered in invertase production and the enzymatic expression underwent strong regulation through glucose concentration in the culture medium and S. cerevisiae CAT-1 presented constitutive behavior for the invertase enzyme.



中文翻译:

碳源对酿酒酵母 FT858 生理学和转化酶活性的影响。

Saccharomyces cerevisiae FT858 是一种发酵效率高的工业酵母菌株,但迄今为止研究很少。这项工作的目的是通过动力学生长参数评估酿酒酵母FT858的生物技术潜力,以及底物浓度对转化酶合成的影响。转化酶具有很高的生物技术潜力,它们通过酵母的生产受到培养基中糖类的强烈影响。与工业酵母参考S. cerevisiae CAT-1相比,S. cerevisiae FT858 具有出色的生物技术潜力,因为它在底物上的甘油产量较低 ( Y GLY/S) 和在 37 °C 下用蔗糖培养的蔗糖乙醇产量增加 10%。底物浓度直接干扰转化酶的产生,酶的表达通过培养基中的葡萄糖浓度进行强烈调节,酿酒酵母CAT-1 呈现出转化酶的组成型行为。

更新日期:2020-07-22
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