Protein dimerization or oligomerization is a key factor in signal transduction processes. It is of great significance to develop novel strategies for investigating protein dimerization in cells and in vivo. Herein, we report a nongenetic proximity-induced fluorescence resonance energy transfer (FRET) strategy based on DNA tetrahedron for visualizing the receptor dimerization. In this nanostrategy, the aptamer serves as the recognition sequence for specifically binding to the receptor monomer. As long as the receptor dimers exist, the proximity switch probes hybridize with each other to induce the FRET between Cy3 and Cy5. Good stability of the DNA tetrahedron in this approach ensures the application value in multiple biological samples. This work provides a new way for developing novel protein dimerization visualization nanostrategies.

中文翻译：

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基于DNA四面体的非遗传邻近诱导FRET策略，用于可视化受体二聚化。

蛋白质二聚或低聚是信号转导过程中的关键因素。开发研究细胞和体内蛋白质二聚化的新策略具有重要意义。。在本文中，我们报告了基于DNA四面体的非遗传邻近诱导荧光共振能量转移（FRET）策略，用于可视化受体二聚体。在该纳米策略中，适体充当特异性结合受体单体的识别序列。只要受体二聚体存在，接近开关探针就会相互杂交，以诱导Cy3和Cy5之间的FRET。DNA四面体在这种方法中的良好稳定性确保了其在多种生物样品中的应用价值。这项工作为开发新型蛋白质二聚化可视化纳米策略提供了新途径。