Centrifugation of boar semen through one layer of 40% colloid (Porcicoll) was previously shown to separate spermatozoa from bacteria without having a detrimental effect on sperm quality. However, some spermatozoa were lost. The purpose of the present study was to determine whether 20% or 30% Porcicoll could be used to recover most of the spermatozoa without impacting on sperm quality. Insemination doses (n = 10) from a commercial boar station were sent to the laboratory at the Swedish University of Agricultural Sciences and processed by Single Layer Centrifugation with 20% and 30% Porcicoll approximately 7 hr after semen collection. The resulting sperm samples and controls were evaluated for sperm quality immediately and again after storage at 16–18°C for 4 and 7 days. Sperm recovery was 94 ± 18% and 87 ± 15% for 20% and 30% Porcicoll, respectively (p > .05). Sperm mitochondrial membrane potential and chromatin integrity were unaffected (p > .05). The proportion of live spermatozoa producing superoxide (9 ± 8%, 7 ± 6% and 3 ± 1%; p < .05), and the proportion of spermatozoa with high stainability DNA (0.68 ± 19%, 0.61 ± 0.22% and 0.96 ± 0.23%; p < .05‐ <0.01), were marginally increased whereas membrane integrity, although high, was lower in the centrifuged samples than in the controls (82 ± 8%, 83 ± 5% versus 92 ± 4%; p < .05). In conclusion, centrifugation through 20% or 30% Porcicoll enables most spermatozoa to be recovered, without having a major effect on sperm quality. These results are encouraging for further studies involving microbiological investigation of the processed samples, and scaling‐up to process larger volumes of boar ejaculates.

中文翻译：

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使用 20% 或 30% Porcicoll 进行单层离心可将大部分精子从样本中分离出来，而不会对精子质量产生不利影响。

以前证明通过一层 40% 胶体 (Porcicoll) 离心公猪精液可以将精子与细菌分离，而不会对精子质量产生不利影响。然而，一些精子丢失了。本研究的目的是确定是否可以使用 20% 或 30% 的 Porcicoll 来回收大部分精子而不影响精子质量。授精剂量 ( n = 10) 从商业公猪站送到瑞典农业科学大学的实验室，并在精液收集后约 7 小时用 20% 和 30% Porcicoll 进行单层离心处理。在 16-18°C 下储存 4 天和 7 天后，立即和再次评估所得精子样本和对照的精子质量。20% 和 30% Porcicoll 的精子回收率分别为 94 ± 18% 和 87 ± 15% ( p  > .05)。精子线粒体膜电位和染色质完整性不受影响 ( p  > .05)。产生超氧化物的活精子的比例（9 ± 8%、7 ± 6% 和 3 ± 1%；p  < .05），以及具有高染色性 DNA 的精子的比例（0.68 ± 19%、0.61 ± 0.22% 和 0.96 ± 0.23%; p < .05- < 0.01)，略有增加，而离心样品中的膜完整性虽然很高，但低于对照组（82 ± 8%、83 ± 5% 与 92 ± 4%；p  < .05）。总之，通过 20% 或 30% 的 Porcicoll 离心可以回收大多数精子，而不会对精子质量产生重大影响。这些结果对进一步的研究令人鼓舞，包括对处理过的样品进行微生物调查，并扩大规模以处理更大量的公猪精液。