Hyperoside, as an active compound, widely exists in a large number of Chinese herbal medicines and has been reported to possess anti‐inflammatory and diuretic properties. However, the effects and underlying mechanisms of hyperoside on periodontitis have not been previously reported. In this study, we found that hyperoside ameliorates symptoms of periodontitis in a rat model, with improvements in alveolar bone resorption, relief of inflammatory infiltration, increase in orderly arrangement of collagen fibers and increase of osteogenic differentiation. In addition, hyperoside promoted proliferation, up‐regulated EdU‐positive cells, decreased cell‐cycle distribution and increased the protein expression of Ki67 and PCNA in rat bone mesenchymal stem cells (rBMSCs), as revealed by Cell Counting Kit‐8, EdU, flow cytometry and western blot analysis. Moreover, hyperoside significantly promoted osteogenic differentiation, as shown by quantitative RT‐PCR, western blot and alizarin red staining assays. Furthermore, hyperoside activated the nuclear factor‐κB (NF‐κB) signaling pathway in rBMSCs, similar to the results observed in vivo. Finally, BMS345541, an inhibitor of the NF‐κB signaling pathway, could reverse the effects of hyperoside on the biological functions in rBMSCs. In conclusion, our results suggest that hyperoside has potential therapeutic properties against periodontitis via promotion of proliferation and osteogenic differentiation of rBMSCs via activation of the NF‐κB signaling pathway.

中文翻译：

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金丝桃苷通过激活 NF-κB 通路促进骨髓间充质干细胞的成骨分化，从而改善大鼠牙周炎。

金丝桃苷作为一种活性化合物，广泛存在于大量中草药中，据报道具有抗炎和利尿作用。然而，金丝桃苷对牙周炎的影响和潜在机制以前没有报道过。在本研究中，我们发现金丝桃苷可以改善大鼠模型牙周炎的症状，改善牙槽骨吸收，缓解炎症浸润，增加胶原纤维的有序排列，增加成骨分化。此外，正如 Cell Counting Kit-8、EdU、流式细胞术和蛋白质印迹分析。此外，金丝桃苷显着促进成骨分化，如定量 RT-PCR、蛋白质印迹和茜素红染色试验所示。此外，金丝桃苷激活了 rBMSCs 中的核因子-κB (NF-κB) 信号通路，与观察到的结果相似体内。最后，NF-κB 信号通路抑制剂 BMS345541 可以逆转金丝桃苷对 rBMSCs 生物学功能的影响。总之，我们的结果表明，金丝桃苷通过激活 NF-κB 信号通路促进 rBMSCs 的增殖和成骨分化，具有治疗牙周炎的潜力。