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Deciphering the role of stress elicitors on the differential modulation of chalcone synthase gene and subsequent production of secondary metabolites in micropropagated Coelogyne ovalis Lindl., a therapeutically important medicinal orchid
South African Journal of Botany ( IF 3.1 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.sajb.2020.06.019
Nutan Singh , Suman Kumaria

Abstract Coelogyne ovalis Lindl. is one of the important medicinal orchid, has been used in traditional medicines for the treatment of various human aliments. The therapetical uses of this orchid may be attributed to the presence of various secondary metabolites ultimately produced via the phenylpropanoid pathway. Amongst the various genes involved in phenylpropanoid pathway, chalcone synthase (CHS) deserves a special mention as it plays a pivotal role in the synthesis of secondary metabolites. In the present research, an attempt has been made to modulate the expressions of CHS transcript and subsequent enhancement in the production of secondary metabolites (phenolics, flavonoids and anthocyanins) and antioxidant activity by the application of biotic (chitosan and yeast extracts) and abiotic (salicylic acid, methyl jasmonate and abscisic acid) stresses to the in vitro-raised plantlets. Amongst the various stress factors taken into consideration, salicylic acid-treated leaves showed the highest tissue-specific expression pattern (13.53-fold at 50 μM), followed by methyl jasmonate (10.80-fold at 100 μM), chitosan (9.04-fold at 100 μM) as compared to control plantlets. The correlation between CHS expression and the presence of secondary metabolites was also studied to find out a suitable condition which would result in higher accumulation of secondary metabolites. Further, elicitations with best three stresses (namely salicylic acid, methyl jasmonate and chitosan) significantly modulated the accumulation of secondary metabolites. Deviations were also detected in terms of phytochemical contents; the salicylic acid-treated leaf tissues showed the highest contents of flavonoids (34.18 mg QE/gm DW) and anthocyanins (24.08 mg / gm FW) whereas phenolic contents were recorded to be highest in pseudobulbs (44.61 mg GAE/ gm DW). The plantlets treated with salicylic acid resulted in enhanced production of secondary metabolites and significantly higher antioxidant activity (DPPH and metal chelating). The present result suggests that stress stimulates CoCHS expression and secondary metabolites production in the treated tissues of C. ovalis.

中文翻译:

解读压力诱发因子对微繁殖 Coelogyne Ovalis Lindl. 中查尔酮合酶基因的差异调节和随后产生的次级代谢产物的作用,一种治疗上重要的药用兰花

摘要 Coelogyne 椭圆形 Lindl。是重要的药用兰花之一,在传统药物中已被用于治疗各种人类的食物。这种兰花的治疗用途可能归因于最终通过苯丙烷途径产生的各种次级代谢物的存在。在参与苯丙烷途径的各种基因中,查尔酮合酶 (CHS) 值得特别提及,因为它在次级代谢物的合成中起着关键作用。在目前的研究中,已尝试通过应用生物(壳聚糖和酵母提取物)和非生物(壳聚糖和酵母提取物)来调节 CHS 转录物的表达,并随后增强次级代谢产物(酚类、黄酮类和花青素)的产生和抗氧化活性(水杨酸,茉莉酸甲酯和脱落酸)对体外培育的小植株的胁迫。在考虑的各种胁迫因素中,水杨酸处理的叶子显示出最高的组织特异性表达模式(50 μM 时的 13.53 倍),其次是茉莉酸甲酯(100 μM 时的 10.80 倍)、壳聚糖(9.04 倍) 100 μM) 与对照植株相比。还研究了 CHS 表达与次级代谢物存在之间的相关性,以找出导致次级代谢物较高积累的合适条件。此外,最好的三种应激(即水杨酸、茉莉酸甲酯和壳聚糖)的诱发显着调节了次生代谢物的积累。在植物化学成分方面也检测到了偏差;水杨酸处理的叶组织显示出最高含量的类黄酮 (34.18 mg QE/gm DW) 和花青素 (24.08 mg/gm FW) 而酚类含量在假鳞茎中最高 (44.61 mg GAE/gm DW)。用水杨酸处理的小植株导致次级代谢产物的产生增加和显着更高的抗氧化活性(DPPH 和金属螯合)。目前的结果表明,压力刺激 CoCHS 表达和次生代谢物在被处理的 C.ovalis 组织中产生。用水杨酸处理的小植株导致次级代谢产物的产生增加和显着更高的抗氧化活性(DPPH 和金属螯合)。目前的结果表明,压力刺激 CoCHS 表达和次生代谢物在被处理的卵圆环虫组织中的产生。用水杨酸处理的小植株导致次级代谢产物的产生增加和显着更高的抗氧化活性(DPPH 和金属螯合)。目前的结果表明,压力刺激 CoCHS 表达和次生代谢物在被处理的 C.ovalis 组织中的产生。
更新日期:2020-07-01
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